Weetman A P, McGregor A M, Wheeler M H, Hall R
Clin Exp Immunol. 1984 May;56(2):330-6.
Several potential sites of thyroid autoantibody production have been investigated using tissue obtained at thyroidectomy for Graves' disease. Spontaneous thyroglobulin and microsomal antibody synthesis was measured using a plaque forming cell assay or a short term culture system coupled to a direct enzyme linked immunoassay (ELISA); these techniques allow the evaluation of lymphocyte populations which have been activated in vivo. Lymphocytes from the cervical lymph nodes, thyroid and bone marrow all contributed to autoantibody production; in contrast no synthesis was demonstrated using cells obtained from the thymus or peripheral blood. Long term culture of the lymphocytes confirmed these findings. The effect of anti-thyroid treatment, with radioiodine, drugs or surgery, on autoantibody production in Graves' disease must be viewed in the light of these results.
利用因格雷夫斯病行甲状腺切除所获得的组织，对甲状腺自身抗体产生的几个潜在部位进行了研究。使用蚀斑形成细胞试验或与直接酶联免疫吸附测定（ELISA）相结合的短期培养系统来测量自发的甲状腺球蛋白和微粒体抗体合成；这些技术能够评估在体内已被激活的淋巴细胞群体。来自颈部淋巴结、甲状腺和骨髓的淋巴细胞均参与自身抗体的产生；相比之下，使用从胸腺或外周血获得的细胞未显示出合成情况。淋巴细胞的长期培养证实了这些发现。鉴于这些结果，必须考虑用放射性碘、药物或手术进行的抗甲状腺治疗对格雷夫斯病自身抗体产生的影响。
