Gardim Sueli, Almeida Carlos E, Takiya Daniela M, Oliveira Jader, Araújo Renato F, Cicarelli Regina M B, da Rosa João A
Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, Sao Paulo, Brazil.
Department of Zoology, Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Infect Genet Evol. 2014 Apr;23:74-9. doi: 10.1016/j.meegid.2014.01.024. Epub 2014 Feb 4.
Multiple fragments of mitochondrial DNA genes (cytochrome b, cytochrome oxidase I, and 16S rDNA) were used to evaluate the phylogenetic relationships among Triatoma melanocephala, Triatoma tibiamaculata, Triatoma vitticeps, and other members of Triatoma brasiliensis subcomplex under a Bayesian framework and maximum parsimony criterion. With the addition of new sequences of T. tibiamaculata and T. vitticeps, Triatoma juazeirensis, Triatoma melanica and the newly sequenced T. melanocephala, the three first sylvatic species, T. melanocephala, T. tibiamaculata and T. vitticeps, were strongly recovered into a clade separate from the other with the remaining Triatoma species from South America, such as the members of T. brasiliensis subcomplex. Panstrongylus megistus was recovered as a sister to T. tibiamaculata, whereas T. vitticeps was a sister to T. melanocephala. This study revealed the non-monophyly of the T. brasiliensis subcomplex, and the polyphyly of Triatoma was reinforced by the placement of these three sylvatic species with Dipetalogaster, Meccus, Mepraia, and Panstrongylus. The results herein shown highlight the need of generic revision in Triatomini.
利用线粒体DNA基因的多个片段（细胞色素b、细胞色素氧化酶I和16S rDNA），在贝叶斯框架和最大简约标准下，评估黑头锥蝽、胫斑锥蝽、饰头锥蝽以及巴西锥蝽亚复合体其他成员之间的系统发育关系。随着胫斑锥蝽和饰头锥蝽新序列、茹阿泽罗锥蝽、黑锥蝽以及新测序的黑头锥蝽的加入，最初的三个野生种类，即黑头锥蝽、胫斑锥蝽和饰头锥蝽，被强烈地聚类为一个与其他种类分开的进化枝，其他种类包括来自南美的锥蝽属其他物种，如巴西锥蝽亚复合体的成员。巨蝽被发现是胫斑锥蝽的姐妹物种，而饰头锥蝽是黑头锥蝽的姐妹物种。本研究揭示了巴西锥蝽亚复合体的非单系性，并且由于这三个野生种类与美洲大锥蝽属、麦锥蝽属、梅普拉锥蝽属和巨蝽属的聚类，锥蝽属的多系性得到了加强。本文所示结果凸显了锥蝽亚科进行类属修订的必要性。
