Revisiting the Hybridization Processes in the Complex (Hemiptera, Triatominae): Reproductive Isolation between and and .

is a species morphologically similar to (which resulted in a synonymization event); despite this similarity, genetic, morphological, and experimental crossbreeding studies confirmed the specific status of . Considering that both species have been reported living in sympatry and that, for a long time, most species of the complex were considered only chromatic variants of , we carried out experimental crosses between and (to confirm whether these species are reproductively isolated) and between and (to assess whether also presents prezygotic isolation with the other species of the complex). Reciprocal experimental crosses were conducted, and weekly, the eggs were collected, counted, and separated in new containers to assess the hatch rate. Neither cross resulted in hybrids, demonstrating that there are pre-zygotic reproductive barriers installed between and the other species of the complex. On the basis of the results above, we demonstrated that is reproductively isolated from and . Furthermore, we suggest that is the species most derived from the complex.