Delgado Luiza Maria Grzyb, Oliveira Jader de, Ravazi Amanda, Madeira Fernanda Fernandez, Reis Yago Visinho Dos, Pinotti Heloisa, Oliveira Ana Beatriz Bortolozo de, Masarin Isabella da Silva, Lilioso Maurício, Folly-Ramos Elaine, Galvão Cleber, Azeredo-Oliveira Maria Tercília Vilela de, Rosa João Aristeu da, Alevi Kaio Cesar Chaboli
Instituto de Biociências, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Rua Dr. Antônio Celso Wagner Zanin, 250, Distrito de Rubião Júnior, Botucatu 18618-689, Brazil.
Laboratório de Entomologia em Saúde Pública, Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo (USP), Av. Dr. Arnaldo 715, São Paulo 01246-904, Brazil.
Insects. 2021 Nov 11;12(11):1015. doi: 10.3390/insects12111015.
is a species morphologically similar to (which resulted in a synonymization event); despite this similarity, genetic, morphological, and experimental crossbreeding studies confirmed the specific status of . Considering that both species have been reported living in sympatry and that, for a long time, most species of the complex were considered only chromatic variants of , we carried out experimental crosses between and (to confirm whether these species are reproductively isolated) and between and (to assess whether also presents prezygotic isolation with the other species of the complex). Reciprocal experimental crosses were conducted, and weekly, the eggs were collected, counted, and separated in new containers to assess the hatch rate. Neither cross resulted in hybrids, demonstrating that there are pre-zygotic reproductive barriers installed between and the other species of the complex. On the basis of the results above, we demonstrated that is reproductively isolated from and . Furthermore, we suggest that is the species most derived from the complex.
是一个在形态上与（这导致了一次同义化事件）相似的物种；尽管有这种相似性，但遗传、形态和实验杂交研究证实了的物种地位。鉴于这两个物种都被报道生活在同域分布中，并且长期以来，复合体的大多数物种仅被视为的色变体，我们进行了和之间的实验杂交（以确认这些物种是否生殖隔离）以及和之间的实验杂交（以评估是否也与复合体的其他物种存在合子前隔离）。进行了正反交实验，并且每周收集、计数卵并将其分开放置在新容器中以评估孵化率。两个杂交组合均未产生杂种，这表明在和复合体的其他物种之间存在合子前生殖障碍。基于上述结果，我们证明了与和生殖隔离。此外，我们认为是复合体中最衍生的物种。
