使用实时环介导等温扩增技术检测犬体内的血小板无浆体。
Detection of Anaplasma platys in dogs using real-time loop-mediated isothermal amplification.
作者信息
Li Hua-tao, Sun Ling-suang, Chen Zhong-ming, Hu Jing-si, Ye Cun-dong, Jia Kun, Wang Heng, Yuan Li-guo, Zhang Gui-hong, Li Shoujun
机构信息
College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Guangzhou 510642, China.
College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Guangzhou 510642, China.
出版信息
Vet J. 2014 Mar;199(3):468-70. doi: 10.1016/j.tvjl.2013.11.012. Epub 2013 Nov 22.
Anaplasma platys is a parasite of canine platelets that causes infectious cyclic thrombocytopenia. In this study, a novel real-time loop-mediated isothermal amplification (RT-LAMP) method was developed to detect A. platys. RT-LAMP primer sets were designed using a citrate synthase gene sequence and the assay was performed at 63 °C for 30 min. No cross-reactivity was observed with other Anaplasma or Ehrlichia spp. and the method exhibited a similar level of sensitivity in detecting the organism in 58 canine blood samples to that of a nested PCR. This RT-LAMP is a rapid and potentially cost-effective method of diagnosing A. platys infection in dogs.
血小板无形体是一种寄生于犬类血小板的寄生虫，可导致感染性周期性血小板减少症。在本研究中，开发了一种新型实时环介导等温扩增（RT-LAMP）方法来检测血小板无形体。使用柠檬酸合酶基因序列设计RT-LAMP引物组，并在63°C下进行30分钟的检测。未观察到与其他无形体或埃立克体属的交叉反应，并且该方法在检测58份犬类血液样本中的该生物体时，与巢式PCR具有相似的灵敏度水平。这种RT-LAMP是一种快速且可能具有成本效益的诊断犬类血小板无形体感染的方法。
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