Hritcu Lucian, Gorgan Lucian Dragos
Department of Biology, "Alexandru Ioan Cuza" University, Iasi, Romania.
Department of Biology, "Alexandru Ioan Cuza" University, Iasi, Romania.
Prog Neuropsychopharmacol Biol Psychiatry. 2014 Jun 3;51:126-32. doi: 10.1016/j.pnpbp.2014.01.016. Epub 2014 Feb 4.
It is known that lipopolysaccharide (LPS) treatment induces neuroinflammation and memory deterioration. One of the mechanisms may be the interference with brain-derived neurotrophic factor (BDNF) gene expression and function. Lipopolysaccharide (3 μg/kg and 10 μg/kg) was unilaterally injected into the substantia nigra of adult male Wistar rats. Pergolide-induced rotational behavior test was employed to validate unilateral damage to the dopamine nigrostriatal neurons. Anxiety-depression-like behaviors were studied by means of elevated plus-maze task and forced swimming test, as animal models of anxiety and depression. Rats given LPS exhibited the following: decrease of the percentage of the time spent and the number of entries in the open arm within elevated plus-maze test and decrease of swimming and increase of immobility times within forced swimming test. In addition, these behaviors are associated with decreased BDNF mRNA expression in rat hippocampus. Taken together, these data indicate that intranigral LPS infusion influences the induction of BDNF mRNA expression within hippocampus which contributes to observed behavioral responses in rats, with relevance for Parkinson's disease conditions.
已知脂多糖（LPS）处理会诱发神经炎症和记忆衰退。其中一种机制可能是对脑源性神经营养因子（BDNF）基因表达和功能的干扰。将脂多糖（3μg/kg和10μg/kg）单侧注射到成年雄性Wistar大鼠的黑质中。采用培高利特诱导的旋转行为试验来验证多巴胺黑质纹状体神经元的单侧损伤。通过高架十字迷宫试验和强迫游泳试验研究焦虑抑郁样行为，作为焦虑和抑郁的动物模型。给予LPS的大鼠表现出以下情况：在高架十字迷宫试验中，开放臂内停留时间百分比和进入次数减少；在强迫游泳试验中，游泳时间减少，不动时间增加。此外，这些行为与大鼠海马中BDNF mRNA表达降低有关。综上所述，这些数据表明黑质内注入LPS会影响海马中BDNF mRNA表达的诱导，这导致了在大鼠中观察到的行为反应，与帕金森病情况相关。
