对五种苏云金芽孢杆菌Vip3A蛋白针对鳞翅目害虫的活性进行筛选。

A screening of five Bacillus thuringiensis Vip3A proteins for their activity against lepidopteran pests.

作者信息

Ruiz de Escudero Iñigo, Banyuls Núria, Bel Yolanda, Maeztu Mireya, Escriche Baltasar, Muñoz Delia, Caballero Primitivo, Ferré Juan

机构信息

Instituto de Agrobiotecnología, CSIC-UPNA, Gobierno de Navarra, Campus Arrosadía, 31192 Mutilva, Navarra, Spain; Laboratorio de Entomología Agrícola y Patología de Insectos, Departamento de Producción Agraria, Universidad Pública de Navarra, 31006 Pamplona, Spain.

Departamento de Genética, Facultad de CC. Biológicas, Universitat de València, 46100 Valencia, Spain.

出版信息

J Invertebr Pathol. 2014 Mar;117:51-5. doi: 10.1016/j.jip.2014.01.006. Epub 2014 Feb 6.

DOI:10.1016/j.jip.2014.01.006

Abstract

Five Bacillus thuringiensis Vip3A proteins (Vip3Aa, Vip3Ab, Vip3Ad, Vip3Ae and Vip3Af) and their corresponding trypsin-activated toxins were tested for their toxicity against eight lepidopteran pests: Agrotis ipsilon, Helicoverpa armigera, Mamestra brassicae, Spodoptera exigua, Spodoptera frugiperda, Spodoptera littoralis, Ostrinia nubilalis and Lobesia botrana. Toxicity was first tested at a high dose at 7 and 10 days. No major differences were found when comparing protoxins vs. trypsin-activated toxins. The proteins that were active against most of the insect species were Vip3Aa, Vip3Ae and Vip3Af, followed by Vip3Ab. Vip3Ad was non-toxic to any of the species tested. Considering the results by insect species, A. ipsilon, S. frugiperda and S. littoralis were susceptible to Vip3Aa, Vip3Ab, Vip3Ae and Vip3Af; S. exigua was susceptible to Vip3Aa and Vip3Ae, and moderately susceptible to Vip3Ab; M. brassicae and L. botrana were susceptible to Vip3Aa, Vip3Ae and Vip3Af; H. armigera was moderately susceptible to Vip3Aa, Vip3Ae and Vip3Af, and O. nubilalis was tolerant to all Vip3 proteins tested, although it showed some susceptibility to Vip3Af. The results obtained will help to design new combinations of insecticidal protein genes in transgenic crops or in recombinant bacteria for the control of insect pests.

摘要

对五种苏云金芽孢杆菌Vip3A蛋白（Vip3Aa、Vip3Ab、Vip3Ad、Vip3Ae和Vip3Af）及其相应的经胰蛋白酶激活的毒素，测试了它们对八种鳞翅目害虫的毒性：小地老虎、棉铃虫、甘蓝夜蛾、甜菜夜蛾、草地贪夜蛾、埃及棉铃虫、欧洲玉米螟和葡萄小卷蛾。首先在第7天和第10天以高剂量测试毒性。比较原毒素和经胰蛋白酶激活的毒素时未发现重大差异。对大多数昆虫种类有活性的蛋白是Vip3Aa、Vip3Ae和Vip3Af，其次是Vip3Ab。Vip3Ad对所测试的任何物种均无毒。按昆虫种类考虑结果，小地老虎、草地贪夜蛾和埃及棉铃虫对Vip3Aa、Vip3Ab、Vip3Ae和Vip3Af敏感；甜菜夜蛾对Vip3Aa和Vip3Ae敏感，对Vip3Ab中度敏感；甘蓝夜蛾和葡萄小卷蛾对Vip3Aa、Vip3Ae和Vip3Af敏感；棉铃虫对Vip3Aa、Vip3Ae和Vip3Af中度敏感，欧洲玉米螟对所有测试的Vip3蛋白均耐受，不过它对Vip3Af表现出一定敏感性。所获得的结果将有助于设计转基因作物或重组细菌中杀虫蛋白基因的新组合，以防治害虫。