棉铃虫对苏云金芽孢杆菌 Vip3Aa、Vip3Ae、Vip3Ad 和 Vip3Af 毒素的敏感性/耐受性的比较分析。
Comparative analysis of the susceptibility/tolerance of Spodoptera littoralis to Vip3Aa, Vip3Ae, Vip3Ad and Vip3Af toxins of Bacillus thuringiensis.
机构信息
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3018 Sfax, Tunisia.
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3018 Sfax, Tunisia; Agronomical Institute of Kef, University of Jendouba, 7100 Kef, Tunisia.
出版信息
J Invertebr Pathol. 2018 Feb;152:30-34. doi: 10.1016/j.jip.2018.01.006. Epub 2018 Jan 31.
The cotton leaf worm Spodoptera littoralis is known for causing serious damages to various crops. In this study, the susceptibility/tolerance of this larvae to four Vip3A (Vip3Aa, Vip3Ae, Vip3Ad and Vip3Af) toxins was investigated. UnlikeVip3Ad which showed no activity to S. littoralis, Vip3Aa, Vip3Ae and Vip3Af exhibited high toxicity to this larva with LC of 228.42 ng/cm, 65.71 ng/cm, and 388.90 ng/cm, respectively. Activation of the 90 kDa Vip3A proteins by S. littoralis larvae juice generated four major bands of sizes 62, 45, 33 and 22 kDa. Binding experiments between biotinylated Vip3A toxins and the brush border membrane vesicles (BBMV) revealed two binding proteins of 55 and 100 kDa with Vip3Aa. Vip3Ae and Vip3Af recognized one single putative receptor of 65 kDa, whereas Vip3Ad did not bind to S. littoralis BBMV. In histopathological observations, Vip3Aa, Vip3Ae and Vip3Af toxins showed approximately similar damages on S. littoralis midgut including rupture and disintegration of epithelial layer and cellular vacuolization. These findings showed that Vip3Aa, Vip3Ae and Vip3Af might be useful for controlling S. littoralis.
棉铃虫（Spodoptera littoralis）以对各种作物造成严重损害而闻名。在本研究中，研究了这些幼虫对四种 Vip3A（Vip3Aa、Vip3Ae、Vip3Ad 和 Vip3Af）毒素的敏感性/耐受性。与对 S. littoralis 没有活性的 Vip3Ad 不同，Vip3Aa、Vip3Ae 和 Vip3Af 对该幼虫表现出高毒性，其 LC 分别为 228.42ng/cm、65.71ng/cm 和 388.90ng/cm。S. littoralis 幼虫汁液激活 90kDa Vip3A 蛋白后，生成大小为 62、45、33 和 22kDa 的四条主要带。生物素化 Vip3A 毒素与刷状缘膜囊泡（BBMV）之间的结合实验表明，Vip3Aa 与 55 和 100kDa 两种结合蛋白结合。Vip3Ae 和 Vip3Af 识别出一种 65kDa 的假定受体，而 Vip3Ad 与 S. littoralis BBMV 不结合。在组织病理学观察中，Vip3Aa、Vip3Ae 和 Vip3Af 毒素对 S. littoralis 中肠表现出相似的损伤，包括上皮层破裂和崩解以及细胞空泡化。这些发现表明，Vip3Aa、Vip3Ae 和 Vip3Af 可能有助于控制 S. littoralis。
Zotero 插件