Johansson G, Joelsson M
Department of Biochemistry, University of Lund, Sweden.
J Chromatogr. 1987 Dec 18;411:161-6. doi: 10.1016/s0021-9673(00)93967-0.
Lactate dehydrogenase from rabbit muscle (EC 1.1.1.27) was partitioned between the liquid phases of aqueous two-phase systems containing two kinds of polymers. The partition was influenced by the use of a dye ligand, Procion Yellow HE-3G, bound to a polymer. The ligand-carrying polymers were poly(ethyleneglycol), poly(vinyl alcohol), ethylhydroxyethylcellulose, hydroxypropyl-starch, Ficoll and dextran. Their influence on the partition of lactate dehydrogenase was studied in systems composed of the following pairs of polymers: dextran-poly(ethylene glycol), dextran-poly(vinyl alcohol), dextran-Ficoll and hydroxypropyl-starch-poly (ethylene glycol). The ratio between the change in the logarithmic partition coefficient of the enzyme, delta log K, caused by the ligand and the logarithm of the partition coefficient of the ligand-polymer, log KL-polymer, was used as a measure of the number of ligand polymers bound per enzyme molecule under saturation conditions. Possible explanations for the observed effects are discussed.
兔肌肉中的乳酸脱氢酶（EC 1.1.1.27）在含有两种聚合物的双水相体系的液相之间进行分配。这种分配受到与聚合物结合的染料配体Procion Yellow HE - 3G的影响。携带配体的聚合物有聚乙二醇、聚乙烯醇、乙基羟乙基纤维素、羟丙基淀粉、聚蔗糖和葡聚糖。在由以下聚合物对组成的体系中研究了它们对乳酸脱氢酶分配的影响：葡聚糖 - 聚乙二醇、葡聚糖 - 聚乙烯醇、葡聚糖 - 聚蔗糖和羟丙基淀粉 - 聚乙二醇。由配体引起的酶的对数分配系数变化（δlog K）与配体 - 聚合物的分配系数对数（log KL - polymer）之间的比率，被用作在饱和条件下每个酶分子结合的配体聚合物数量的度量。讨论了对观察到的效应的可能解释。
