Chowdhary Sheemona, Bhattacharyya Rajasri, Banerjee Dibyajyoti
Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh 160012, India.
Department of Biotechnology, Maharishi Markandeshwar University, Mullana, Ambala, Haryana, India.
Clin Chim Acta. 2014 Apr 20;431:66-76. doi: 10.1016/j.cca.2014.01.024. Epub 2014 Feb 7.
Acute organophosphorus poisoning continues to be a detrimental problem and a potential cause of mortality especially in developing countries. Inhibition of acetylcholinesterase enzyme is the main mechanism of toxicity of such pesticides and measurement of acetylcholinesterase activity is the commonly used laboratory diagnosis approved for the purpose. It is now proved beyond any doubt that early intervention is beneficial for cases of acute organophosphorus poisoning and, therefore, considerable current interest has been generated for development of point of care testing tool for screening of the same. However, to the best of our knowledge so far the matter is not reviewed from the view of point of care testing tool development. In this paper, this subject is reviewed highlighting the methodological aspects and point of care testing tool development in the context of organophosphorus poisoning.
急性有机磷中毒仍然是一个有害问题，尤其是在发展中国家，它是潜在的致死原因。抑制乙酰胆碱酯酶是这类农药毒性的主要机制，测定乙酰胆碱酯酶活性是为此目的而批准的常用实验室诊断方法。现在毫无疑问地证明，早期干预对急性有机磷中毒病例有益，因此，目前人们对开发用于筛查该病的即时检测工具产生了浓厚兴趣。然而，据我们所知，迄今为止尚未从即时检测工具开发的角度对此事进行综述。本文对这一主题进行了综述，重点介绍了有机磷中毒背景下的方法学方面和即时检测工具的开发。
