Chen Yuan-Wu, Lin Gu-Jiun, Hueng Dong-Yuan, Huang Shing-Hwa, Chia Wei-Tso, Shieh Yi-Shing, Ma Kuo-Hsing, Sytwu Huey-Kang
School of Dentistry, National Defense Medical Center, Taipei, Taiwan.
Department of Biology and Anatomy, National Defense Medical Center, Taipei, Taiwan.
Planta Med. 2014 Mar;80(4):255-61. doi: 10.1055/s-0033-1360315. Epub 2014 Feb 7.
Advanced oral cancer has a poor prognosis because of the lack of an effective treatment. We explored the efficiency of combined treatment with triptolide and ionizing radiation for treating oral cancer. Human tongue cancer cells were treated with triptolide, ionizing radiation, or triptolide plus ionizing radiation. Cell proliferation, cell cycle arrest, and apoptotic influences were analyzed by FACS and immunohistochemistry. Tumor potency was examined in an in vivo human tongue cancer cells xenograft mouse model. Our results demonstrated that triptolide caused a marked reduction in colony number that was further enhanced with increasing doses of ionizing radiation. Triptolide increased apoptosis and decreased the expression of anti-apoptotic proteins. In vivo, combination treatment synergistically reduced tumor weight and volume possibly via the induction of apoptosis and reduction in anti-apoptotic protein expression. In conclusion, triptolide plus ionizing radiation treatment had synergistic anti-tumor effects, especially in vivo, and may be a promising combined modality therapy for advanced oral cancer.
由于缺乏有效的治疗方法，晚期口腔癌的预后较差。我们探讨了雷公藤甲素与电离辐射联合治疗口腔癌的疗效。用人舌癌细胞分别接受雷公藤甲素、电离辐射或雷公藤甲素加电离辐射处理。通过流式细胞术和免疫组织化学分析细胞增殖、细胞周期阻滞和凋亡影响。在体内人舌癌细胞异种移植小鼠模型中检测肿瘤效能。我们的结果表明，雷公藤甲素导致集落数量显著减少，随着电离辐射剂量增加，这种减少进一步增强。雷公藤甲素增加凋亡并降低抗凋亡蛋白的表达。在体内，联合治疗可能通过诱导凋亡和降低抗凋亡蛋白表达协同降低肿瘤重量和体积。总之，雷公藤甲素加电离辐射治疗具有协同抗肿瘤作用，尤其是在体内，可能是晚期口腔癌一种有前景的联合治疗方式。
