Wangorsch Andrea, Jamin Annette, Foetisch Kay, Malczyk Anna, Reuter Andreas, Vierecke Sabine, Schülke Stefan, Bartel Detlef, Mahler Vera, Lidholm Jonas, Vieths Stefan, Scheurer Stephan
Paul-Ehrlich-Institut, Division of Allergology, Langen, Germany.
Mol Nutr Food Res. 2015 Mar;59(3):582-92. doi: 10.1002/mnfr.201300620. Epub 2014 Feb 8.
The aim was to investigate the potential contribution of a major birch pollen Bet v 1-homologous allergen to birch pollen-associated tomato fruit allergy.
Two isoforms of a Bet v 1-homologous protein (designated Sola l 4) from tomato fruit were identified by cDNA-cloning and produced as recombinant proteins. Allergen-specific IgE levels to tomato, birch pollen, Bet v 1, and Sola l 4 were determined in birch pollen allergic patients with allergy or tolerance to tomato. Sola l 4 was recognized in 76% of birch/tomato allergic patients, while tomato- and Bet v 1-specific IgE was detectable in 64% and 81% of sera. Almost all patients sensitized to Bet v 1 reacted with Sola l 4. Both Sola l 4 isoforms displayed allergenic potency and IgE-cross-reactivity with Bet v 1 as investigated by competitive ELISA and in vitro mediator release assay. Nevertheless, the reactivity pattern of patients' sera was diverse.
Sola l 4, a novel pathogenesis related-10 protein, qualifies as major allergen in tomato fruits. Data suggest Sola l 4 as class II allergen. IgE-testing using Sola l 4 showed low clinical specificity, but high sensitivity in tomato allergic patients and will further improve component-resolved allergy diagnosis.
目的是研究一种主要的桦树花粉Bet v 1同源变应原对桦树花粉相关的番茄果实过敏的潜在作用。
通过cDNA克隆鉴定了番茄果实中一种Bet v 1同源蛋白的两种亚型（命名为Sola l 4），并将其制备为重组蛋白。在对番茄过敏或耐受的桦树花粉过敏患者中测定了对番茄、桦树花粉、Bet v 1和Sola l 4的变应原特异性IgE水平。76%的桦树/番茄过敏患者可识别出Sola l 4，而64%和81%的血清中可检测到番茄和Bet v 1特异性IgE。几乎所有对Bet v 1致敏的患者都对Sola l 4有反应。通过竞争性ELISA和体外介质释放试验研究发现，两种Sola l 4亚型均表现出变应原活性以及与Bet v 1的IgE交叉反应性。然而，患者血清的反应模式各不相同。
Sola l 4是一种新型的病程相关蛋白10，可作为番茄果实中的主要变应原。数据表明Sola l 4为II类变应原。使用Sola l 4进行IgE检测在番茄过敏患者中显示出较低的临床特异性，但具有较高的敏感性，将进一步改善组分分辨变应原诊断。
