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Vig r 6是来自绿豆（Vigna radiata）芽苗的细胞分裂素特异性结合蛋白，它与Bet v 1相关过敏原发生交叉反应，并结合桦树花粉过敏患者血清中的IgE。

Vig r 6, the cytokinin-specific binding protein from mung bean (Vigna radiata) sprouts, cross-reacts with Bet v 1-related allergens and binds IgE from birch pollen allergic patients' sera.

作者信息

Guhsl Eva Elisabeth, Hofstetter Gerlinde, Hemmer Wolfgang, Ebner Christof, Vieths Stefan, Vogel Lothar, Breiteneder Heimo, Radauer Christian

机构信息

Department of Pathophysiology and Allergy Research, Medical University of Vienna, Austria.

出版信息

Mol Nutr Food Res. 2014 Mar;58(3):625-34. doi: 10.1002/mnfr.201300153. Epub 2013 Sep 1.

DOI:10.1002/mnfr.201300153
PMID:23996905
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4135424/
Abstract

SCOPE

Birch pollen associated allergy to mung bean sprouts is caused by cross-reactivity between the birch pollen allergen Bet v 1 and the mung bean allergen Vig r 1. We aimed to determine the allergenicity of the cytokinin-specific binding protein from mung bean (Vig r 6), another allergen related to Bet v 1 with only 31% sequence identity.

METHODS AND RESULTS

Bet v 1, Gly m 4, Vig r 1, and Vig r 6 were produced in Escherichia coli. In an ELISA, 73 and 32% of Bet v 1-sensitized birch-allergic patients' sera (n = 60) showed IgE binding to Vig r 1 and Vig r 6, respectively. Of 19 patients who reported allergic reactions or had positive prick-to-prick tests to mung bean sprouts, 79% showed IgE binding to Vig r 1 and 63% showed IgE binding to Vig r 6. Bet v 1 completely inhibited IgE binding to both mung bean allergens. Vig r 6 showed partial cross-reactivity with Vig r 1 and activated basophils sensitized with mung bean allergic patients' sera.

CONCLUSION

We demonstrated IgE cross-reactivity despite low sequence identity between Vig r 6 and other Bet v 1-related allergens. Thus, IgE binding to Vig r 6 may contribute to birch pollinosis-associated mung bean sprout allergy.

摘要

范围

桦树花粉相关的绿豆芽过敏是由桦树花粉过敏原Bet v 1与绿豆过敏原Vig r 1之间的交叉反应引起的。我们旨在确定绿豆细胞分裂素特异性结合蛋白（Vig r 6）的致敏性，Vig r 6是另一种与Bet v 1相关的过敏原，其序列同一性仅为31%。

方法与结果

Bet v 1、Gly m 4、Vig r 1和Vig r 6在大肠杆菌中产生。在酶联免疫吸附测定（ELISA）中，73%和32%的对Bet v 1致敏的桦树过敏患者血清（n = 60）分别显示IgE与Vig r 1和Vig r 6结合。在19名报告对绿豆芽有过敏反应或点刺试验呈阳性的患者中，79%显示IgE与Vig r 1结合，63%显示IgE与Vig r 6结合。Bet v 1完全抑制IgE与两种绿豆过敏原的结合。Vig r 6与Vig r 1显示部分交叉反应，并激活用绿豆过敏患者血清致敏的嗜碱性粒细胞。

结论

尽管Vig r 6与其他Bet v 1相关过敏原之间的序列同一性较低，但我们仍证明了IgE交叉反应性。因此，IgE与Vig r 6的结合可能导致与桦树花粉症相关的绿豆芽过敏。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/887a/4135424/fba92bbe3319/emss-54711-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/887a/4135424/a366ddfd718f/emss-54711-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/887a/4135424/6060535e00bf/emss-54711-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/887a/4135424/180741e85a39/emss-54711-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/887a/4135424/55576b1a34c1/emss-54711-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/887a/4135424/ca8a5a9db7d9/emss-54711-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/887a/4135424/fba92bbe3319/emss-54711-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/887a/4135424/a366ddfd718f/emss-54711-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/887a/4135424/6060535e00bf/emss-54711-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/887a/4135424/180741e85a39/emss-54711-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/887a/4135424/55576b1a34c1/emss-54711-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/887a/4135424/ca8a5a9db7d9/emss-54711-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/887a/4135424/fba92bbe3319/emss-54711-f0006.jpg

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