Kimple Michelle E, Brill Allison L, Pasker Renee L
University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
Curr Protoc Protein Sci. 2013 Sep 24;73:9.9.1-9.9.23. doi: 10.1002/0471140864.ps0909s73.
Addition of an affinity tag is a useful method for differentiating recombinant proteins expressed in bacterial and eukaryotic expression systems from the background of total cellular proteins, as well as for detecting protein-protein interactions. This overview describes the historical basis for the development of affinity tags, affinity tags that are commonly used today, how to choose an appropriate affinity tag for a particular purpose, and several recently developed affinity tag technologies that may prove useful in the near future.
添加亲和标签是一种有用的方法，可用于从总细胞蛋白背景中区分在细菌和真核表达系统中表达的重组蛋白，以及检测蛋白质-蛋白质相互作用。本综述描述了亲和标签发展的历史基础、当今常用的亲和标签、如何为特定目的选择合适的亲和标签，以及几种可能在不久的将来证明有用的最新开发的亲和标签技术。
