基于实时荧光定量PCR的拷贝数变异定量分析。
Quantitative analysis of copy number variants based on real-time LightCycler PCR.
作者信息
Ma Lijiang, Chung Wendy K
机构信息
Department of Pediatrics, Columbia University, New York, New York.
Department of Medicine, Columbia University, New York, New York.
出版信息
Curr Protoc Hum Genet. 2014 Jan 21;80:7.21.1-7.21.8. doi: 10.1002/0471142905.hg0721s80.
Abstract
Quantitative real-time PCR is PCR visualized in real time by the use of fluorescent or intercalating dyes, which are employed to measure gene expression or gene quantification including contiguous gene deletions or duplications. A simple method is described here to quantify DNA copy number from human samples.
摘要
定量实时聚合酶链反应（Quantitative real-time PCR）是通过使用荧光染料或嵌入染料对聚合酶链反应进行实时可视化，这些染料用于测量基因表达或基因定量，包括相邻基因的缺失或重复。本文描述了一种从人类样本中定量DNA拷贝数的简单方法。
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