Enna S J, McCarson Kenneth E
University of Kansas Medical Center, Kansas City, Kansas.
Curr Protoc Pharmacol. 2013 Dec 2;63:1.7.1-1.7.20. doi: 10.1002/0471141755.ph0107s63.
Described in this unit are ligand-binding assays for GABAA , GABAB , and the homomeric ρ GABAA (formerly GABAC ) receptor recognition sites in brain tissue. Although GABA binding sites are present in peripheral organs, most research is directed toward examining these receptors in the CNS. These assays may also be used to determine the affinity of an unlabeled compound for the GABA binding sites. Excluded from the unit are ligand-binding assays for other components of the GABAA receptor complex, such as the benzodiazepine or ion-channel binding sites.
本单元介绍了针对脑组织中GABAA、GABAB和同聚体ρ GABAA（原GABAC）受体识别位点的配体结合分析方法。尽管外周器官中也存在GABA结合位点，但大多数研究都集中在中枢神经系统中对这些受体的研究。这些分析方法也可用于确定未标记化合物对GABA结合位点的亲和力。本单元不包括对GABAA受体复合物其他成分（如苯二氮䓬或离子通道结合位点）的配体结合分析。
