远程缺血预处理及N-乙酰半胱氨酸联合远程缺血预处理在大鼠肝脏缺血再灌注损伤模型中的作用。

The effects of remote ischemic preconditioning and N-acetylcysteine with remote ischemic preconditioning in rat hepatic ischemia reperfusion injury model.

作者信息

Uysal Ali Ihsan, Ocmen Elvan, Akan Mert, Ozkardesler Sevda, Ergur Bekir Ugur, Guneli Ensari, Kume Tuncay, Koca Uğur, Unal Togrul Belgin

机构信息

Department of Anesthesiology and Reanimation, Dokuz Eylul University Medical School, Inciralti, 35340 Izmir, Turkey.

Department of Histology and Embryology, University Medical School, 35340 Izmir, Turkey.

出版信息

Biomed Res Int. 2014;2014:892704. doi: 10.1155/2014/892704. Epub 2014 Jan 8.

DOI:10.1155/2014/892704

PMID:24511549

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3910499/

Abstract

BACKGROUND

Remote ischemic preconditioning (RIP) and pharmacological preconditioning are the effective methods that can be used to prevent ischemia reperfusion (IR) injury. The aim of this study was to evaluate the effects of RIP and N-Acetylcysteine (NAC) with RIP in the rat hepatic IR injury model.

MATERIALS AND METHODS

28 rats were divided into 4 groups. Group I (sham): only laparotomy was performed. Group II (IR): following 30 minutes of hepatic pedicle occlusion, 4 hours of reperfusion was performed. Group III (RIP + IR): following 3 cycles of RIP, hepatic IR was performed. Group IV (RIP + NAC + IR): following RIP and intraperitoneal administration of NAC (150 mg/kg), hepatic IR was performed. All the rats were sacrificed after blood samples were taken for the measurements of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels and liver was processed for conventional histopathology.

RESULTS

The hepatic histopathological injury scores of RIP + IR and RIP + NAC + IR groups were significantly lower than IR group (P = 0.006, P = 0.003, resp.). There were no significant differences in AST and ALT values between the IR, RIP + IR, and RIP + NAC + IR groups.

CONCLUSIONS

In the present study, it was demonstrated histopathologically that RIP and RIP + NAC decreased hepatic IR injury significantly.

摘要

背景

远程缺血预处理（RIP）和药物预处理是可用于预防缺血再灌注（IR）损伤的有效方法。本研究的目的是评估RIP以及RIP联合N-乙酰半胱氨酸（NAC）在大鼠肝脏IR损伤模型中的作用。

材料与方法

将28只大鼠分为4组。I组（假手术组）：仅进行剖腹手术。II组（IR组）：肝蒂阻断30分钟后，进行4小时再灌注。III组（RIP + IR组）：进行3个周期的RIP后，进行肝脏IR。IV组（RIP + NAC + IR组）：进行RIP并腹腔注射NAC（150 mg/kg）后，进行肝脏IR。采集血样测定天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）水平后，处死所有大鼠，并对肝脏进行常规组织病理学检查。