Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, P. R. China.
ACS Appl Mater Interfaces. 2014 Mar 12;6(5):3189-94. doi: 10.1021/am405864z. Epub 2014 Feb 18.
DNA-functionalized quantum dots (QDs) are powerful tools for biosensing and bioimaging applications. Facile labeling methods with good fluorescence properties are desirable for the development of DNA-functionalized QDs. In this article, we describe a novel and simple approach that leads to the synthesis of DNA-functionalized CdTe:Zn(2+) QDs in one step. It is the first time that DNA-functionalized QDs have been prepared using sodium tellurite as the tellurium source by a hydrothermal method. This approach will greatly reduce the synthesis time (only about 1 h) and simplify the synthesis process as well as reduce the complexity of the required experimental techniques. The as-prepared QDs exhibit high quantum yield, small size, and low toxicity. UV-vis spectra and FTIR characterization proved that the abundance of DNA on the surface of the QDs increased with the increase in the concentration of the feed DNA. Most importantly, these QDs functionalized with DNA have great potential to bind specifically to DNA, protein, and cell surface receptors.
DNA 功能化量子点（QDs）是生物传感和生物成像应用的有力工具。对于 DNA 功能化 QDs 的发展，需要具有良好荧光性能的简便标记方法。本文描述了一种新的简单方法，可一步合成 DNA 功能化的 CdTe:Zn(2+) QDs。这是首次通过水热法使用亚碲酸钠作为碲源来制备 DNA 功能化的 QDs。这种方法将大大缩短合成时间（仅约 1 小时），简化合成过程，并降低所需实验技术的复杂性。所制备的 QDs 具有高量子产率、小尺寸和低毒性。紫外-可见光谱和傅里叶变换红外光谱（FTIR）表征证明，随着进料 DNA 浓度的增加，QDs 表面上的 DNA 丰度增加。最重要的是，这些用 DNA 功能化的 QDs 具有与 DNA、蛋白质和细胞表面受体特异性结合的巨大潜力。
