Zucker M L, Dube S, Prusoff W H
Department of Pharmacology, Yale University Medical School, New Haven, Connecticut.
Virus Res. 1988 Feb;9(2-3):221-32. doi: 10.1016/0168-1702(88)90032-9.
Treatment of herpes simplex virus type 1 (HSV-1) infected Vero cells with 5'-amino-5'-deoxythymidine (AdThd) causes a 65% reduction in the amount of total viral RNA present at late times of infection. This decrease is apparent as early as 6 hours post infection, affecting the levels of viral cytoplasmic polyadenylated (poly(A+] species to a greater extent than non-polyadenylated (poly(A-] RNA. Cytoplasmic viral poly(A+) RNA is present in AdThd-treated cultures at only 10% control poly(A+) levels, yet there is no evidence of a direct inhibition of RNA polyadenylation. Underrepresentation of RNA species in the cytoplasm is not sequence-specific. The results suggest that the antiviral activity of AdThd may involve not only incorporation into viral DNA, with a resultant decrease in viral transcription, but also perturbation of the relative amounts of viral poly(A+) RNA and poly(A-) RNA.
用5'-氨基-5'-脱氧胸苷（AdThd）处理单纯疱疹病毒1型（HSV-1）感染的非洲绿猴肾细胞（Vero细胞），可使感染后期的病毒RNA总量减少65%。这种减少在感染后6小时就很明显，对病毒细胞质多聚腺苷酸化（poly(A+)）的影响程度大于非多聚腺苷酸化（poly(A-)）的RNA。在经AdThd处理的培养物中，细胞质病毒poly(A+) RNA的含量仅为对照poly(A+)水平的10%，但没有证据表明RNA多聚腺苷酸化受到直接抑制。细胞质中RNA种类的表达不足并非序列特异性的。结果表明，AdThd的抗病毒活性可能不仅涉及掺入病毒DNA，从而导致病毒转录减少，还涉及扰乱病毒poly(A+) RNA和poly(A-) RNA的相对含量。
