Otto M J, Lee J J, Prusoff W H
Antiviral Res. 1982 Oct;2(5):267-81. doi: 10.1016/0166-3542(82)90050-x.
Exposure of herpes simplex virus type 1 (HSV-1)-infected Vero cells to the nucleoside analogues 5-iodo-5'-amino-2',5'-dideoxyuridine (AIdUrd), 5-iodo-2'-deoxyuridine (IdUrd) or 5'-amino-2',5'-dideoxythymidine (5'-AdThd) resulted in altered expression of HSV-1-induced proteins. Infected cell proteins (ICPs) synthesized in the presence of the nucleoside analogues were compared by sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis to ICPs from non-drug-treated cells and it was found that there was no effect on HSV-1-induced alpha proteins but beta and gamma proteins were reduced as much as 60%. There were three exceptions: ICP 35 (Mr = 46,000) and ICP 39 (Mr = 36,000) were not reduced and ICP 36 (Mr = 42,000) was increased during drug treatment. Progeny virions were isolated from drug-treated infected Vero cells and were compared to progeny isolated from control cells with respect to their polypeptide make-up and for their ability to induce HSV-1 proteins in non-drug-treated Vero cells. The progeny virus from drug-treated cells exhibited altered protein patterns on SDS-polyacrylamide gels with respect to control HSV-1. The progeny virions from AIdUrd- or IdUrd- but not from 5'-AdThd-treated cells were defective in their abilities to induce proteins upon subsequent infection of non-drug-treated Vero cells. Two unusual phosphoproteins were detected; one with an apparent molecular weight of 30,000 was induced by progeny virus from AIdUrd-treated cells and another at approximately 69,000 was induced by progeny virus from 5'-AdThd-treated cells.
将单纯疱疹病毒1型（HSV-1）感染的非洲绿猴肾细胞（Vero细胞）暴露于核苷类似物5-碘-5'-氨基-2'，5'-二脱氧尿苷（AIdUrd）、5-碘-2'-脱氧尿苷（IdUrd）或5'-氨基-2'，5'-二脱氧胸苷（5'-AdThd），会导致HSV-1诱导蛋白的表达发生改变。通过十二烷基硫酸钠（SDS）聚丙烯酰胺凝胶电泳，将在核苷类似物存在下合成的感染细胞蛋白（ICP）与未用药物处理细胞的ICP进行比较，发现对HSV-1诱导的α蛋白没有影响，但β和γ蛋白减少了多达60%。有三个例外：ICP 35（分子量=46,000）和ICP 39（分子量=36,000）没有减少，并且在药物处理期间ICP 36（分子量=42,000）增加。从用药物处理的感染Vero细胞中分离出子代病毒颗粒，并将其与从对照细胞中分离出的子代病毒颗粒在多肽组成以及在未用药物处理的Vero细胞中诱导HSV-1蛋白的能力方面进行比较。与对照HSV-1相比，来自用药物处理细胞的子代病毒在SDS-聚丙烯酰胺凝胶上呈现出改变的蛋白模式。来自AIdUrd或IdUrd处理细胞而非5'-AdThd处理细胞的子代病毒颗粒在随后感染未用药物处理的Vero细胞时诱导蛋白的能力存在缺陷。检测到两种异常的磷蛋白；一种表观分子量为30,000的磷蛋白由来自AIdUrd处理细胞的子代病毒诱导，另一种约69,000的磷蛋白由来自5'-AdThd处理细胞的子代病毒诱导。
