Sasaki J, Sawamura D, Kitagawa M, Ahsan C R, Lü C, Masumori J
Dept. of Bacteriology, Hirosaki University School of Medicine.
Gan To Kagaku Ryoho. 1988 Apr;15(4 Pt 1):649-53.
FITC-Con A staining as a rapid diagnostic method for tumor cells was applied to the tumors smeared on glass slide and section specimens to evaluate the reactivity with FITC-Con A. Good staining results were obtained in smear specimens with clear fluorescence on the membrane of tumor cells. Con A and LCH lectins bound well with tumor cells to produce strong fluorescence in comparison with PEA and DBA. It indicates that tumor cells expressed dominantly the receptors of alpha-D-glucose and alpha-D-mannose sugar chain on the membrane of tumor cells. From these results it was concluded that FITC-Con A staining method applied to smear specimens is more advantageous in the rapidity and the simplicity for tumor cell diagnosis than section specimen method.
将异硫氰酸荧光素标记的刀豆球蛋白A（FITC-Con A）染色作为肿瘤细胞的快速诊断方法，应用于涂在载玻片上的肿瘤涂片和切片标本，以评估其与FITC-Con A的反应性。在肿瘤细胞涂片标本中获得了良好的染色结果，肿瘤细胞膜上有清晰的荧光。与豌豆凝集素（PEA）和荆豆凝集素（DBA）相比，Con A和LCH凝集素与肿瘤细胞结合良好，产生强烈荧光。这表明肿瘤细胞在细胞膜上主要表达α-D-葡萄糖和α-D-甘露糖糖链的受体。从这些结果可以得出结论，应用于涂片标本的FITC-Con A染色方法在肿瘤细胞诊断的快速性和简便性方面比切片标本方法更具优势。
