Chung Hsiang-Yu, Kuo Wei-Cheng, Cheng Yu-Hsiang, Yu Che-Hang, Chia Shih-Hsuan, Lin Cheng-Yung, Chen Jie-Shin, Tsai Huai-Jen, Fedotov Andrey B, Ivanov Anatoly A, Zheltikov Aleksei M, Sun Chi-Kuang
Opt Express. 2013 Dec 16;21(25):31604-14. doi: 10.1364/OE.21.031604.
In this paper, we examine the performance of a Blu-ray disk (BD) aspheric lens as the objective of a miniaturized scanning nonlinear optical microscope. By combining a single 2D micro-electro mechanical system (MEMS) mirror as the scanner and with different tube lens pairs, the field of view (FOV) of the studied microscope varies from 59 μm × 93 μm up to 178 μm × 280 μm, while the corresponding lateral resolution varies from 0.6 μm to 2 μm for two-photon fluorescence (2PF) signals. With a 34/s video frame rate, in vivo dynamic observation of zebrafish heartbeat through 2PF of the excited green fluorescence protein (GFP) is demonstrated.
在本文中，我们研究了蓝光光盘（BD）非球面透镜作为小型化扫描非线性光学显微镜物镜的性能。通过将单个二维微机电系统（MEMS）镜用作扫描仪并结合不同的管透镜对，所研究显微镜的视场（FOV）从59μm×93μm变化到178μm×280μm，而对于双光子荧光（2PF）信号，相应的横向分辨率从0.6μm变化到2μm。在34帧/秒的视频帧率下，展示了通过激发绿色荧光蛋白（GFP）的2PF对斑马鱼心跳进行体内动态观察。
