Kostamovaara Juha, Tenhunen Jussi, Kögler Martin, Nissinen Ilkka, Nissinen Jan, Keränen Pekka
Opt Express. 2013 Dec 16;21(25):31632-45. doi: 10.1364/OE.21.031632.
A Raman spectrometer technique is described that aims at suppressing the fluorescence background typical of Raman spectra. The sample is excited with a high power (65W), short (300ps) laser pulse and the time position of each of the Raman scattered photons with respect to the excitation is measured with a CMOS SPAD detector and an accurate time-to-digital converter at each spectral point. It is shown by means of measurements performed on an olive oil sample that the fluorescence background can be greatly suppressed if the sample response is recorded only for photons coinciding with the laser pulse. A further correction in the residual fluorescence baseline can be achieved using the measured fluorescence tails at each of the spectral points.
本文描述了一种拉曼光谱仪技术，其目的是抑制拉曼光谱中典型的荧光背景。用高功率（65W）、短脉冲（300ps）的激光脉冲激发样品，并用CMOS单光子雪崩二极管探测器和精确的时间数字转换器在每个光谱点测量每个拉曼散射光子相对于激发的时间位置。通过对橄榄油样品进行的测量表明，如果仅记录与激光脉冲同时出现的光子的样品响应，则可以大大抑制荧光背景。利用在每个光谱点测量的荧光拖尾，可以对残余荧光基线进行进一步校正。
