Chew Yi Vee, Holmes Andrew J, Cliff John B
School of Molecular Bioscience, University of Sydney, Sydney, NSW, Australia.
Methods Mol Biol. 2014;1096:133-46. doi: 10.1007/978-1-62703-712-9_11.
NanoSIMS combines high-resolution imaging and mass spectrometry with simultaneous collection of up to seven different masses, providing an invaluable technique for determining the isotopic and elemental composition in microscopic target samples. It has been used in varying fields, from studying the elemental composition of mineral samples to tracking cell uptake of isotope-labelled substrates. In combination with in situ hybridization techniques, NanoSIMS offers a powerful method of linking metabolic capacity to phylogenetic identity in cell samples. Here, we describe methods and considerations for microbial sample preparation, visualization, and analysis using NanoSIMS.
纳米二次离子质谱仪（NanoSIMS）将高分辨率成像和质谱分析相结合，可同时收集多达七种不同的质量数，为确定微观目标样品中的同位素和元素组成提供了一种极为重要的技术。它已被应用于多个不同领域，从研究矿物样品的元素组成到追踪细胞对同位素标记底物的摄取。与原位杂交技术相结合，NanoSIMS为将细胞样品中的代谢能力与系统发育特征联系起来提供了一种强大的方法。在此，我们描述了使用NanoSIMS进行微生物样品制备、可视化和分析的方法及注意事项。
