Chen Jiaxi, Jun Li, Shiyong Chen, Li Hou, Zhu Ming, Shen Bo
Department of Clinical Laboratory, Taizhou Hospital of Zhejiang Province, Affiliated Hospital of Wenzhou Medical College, Taizhou, Zhejiang Province, China.
Clin Exp Med. 2015 Feb;15(1):25-30. doi: 10.1007/s10238-014-0274-9. Epub 2014 Feb 11.
Immune system activation is known to be involved in the progression of rheumatoid arthritis (RA). The aim of this work was to study the imbalance expressions of indoleamine 2,3-dioxygenase (IDO) and tryptophanyl-tRNA synthetase (TTS) with RA patients. Forty-nine RA patients and 49 healthy controls were studied. The expressions of IDO and TTS were analyzed by real-time quantitative polymerase chain reaction and flow cytometry in peripheral blood mononuclear cells. The expression of TTS mRNA increased significantly in RA patients when compared with healthy controls and correlated with erythrocyte sedimentation rate (r = 0.424, P < 0.01). In addition, we found TTS increased significantly mainly in CD3(+) T cells in rheumatoid arthritis group. Increased TTS expressions from CD3(+) T cells might link to a pathogenic mechanism involved in increasing survival of autoreactive T cells in RA patients. Determination of expressions of TTS may provide a better understanding of progression of the disease.
已知免疫系统激活与类风湿关节炎（RA）的进展有关。这项工作的目的是研究RA患者中吲哚胺2,3-双加氧酶（IDO）和色氨酰-tRNA合成酶（TTS）的表达失衡情况。对49例RA患者和49例健康对照进行了研究。通过实时定量聚合酶链反应和流式细胞术分析外周血单个核细胞中IDO和TTS的表达。与健康对照相比，RA患者中TTS mRNA的表达显著增加，且与红细胞沉降率相关（r = 0.424，P < 0.01）。此外，我们发现类风湿关节炎组中TTS主要在CD3(+) T细胞中显著增加。CD3(+) T细胞中TTS表达的增加可能与RA患者自身反应性T细胞存活增加所涉及的致病机制有关。TTS表达的测定可能有助于更好地理解该疾病的进展。
