Müller-Sienerth Nicole, Crosnier Cécile, Wright Gavin J, Staudt Nicole
Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
Methods Mol Biol. 2014;1131:229-40. doi: 10.1007/978-1-62703-992-5_14.
Antibodies are an integral part of biological and medical research. In addition, immunoglobulins are used in many diagnostic tests and are becoming increasingly important in the therapy of diseases. To express antibodies recombinantly, the immunoglobulin heavy and light chains are usually cloned into two different expression plasmids. Here, we describe a method for recombinant antibody expression from a single plasmid.
抗体是生物学和医学研究不可或缺的一部分。此外，免疫球蛋白被用于许多诊断测试，并且在疾病治疗中变得越来越重要。为了重组表达抗体，免疫球蛋白重链和轻链通常被克隆到两个不同的表达质粒中。在此，我们描述一种从单个质粒进行重组抗体表达的方法。
