Mita H, Yui Y, Yasueda H, Shida T
Clinical Research Center for Rheumato-Allergology, National Sagamihara Hospital, Kanagawa, Japan.
Int Arch Allergy Appl Immunol. 1988;85(4):422-7. doi: 10.1159/000234545.
Addition of deuterium oxide (D2O), 6-36%, resulted in a dose-dependent increase in allergen- or anti-IgE-induced leukotriene C4 (LTC4) generation from human basophils. In the presence of 36% D2O, the enhancement was 260 +/- 135% for allergen stimulation and 480 +/- 152% for anti-IgE stimulation as compared with the control incubated in normal buffer. The increasing effect of D2O on LTC4 generation from basophils was completely reversed by washing the cells before incubation with allergen. Vinblastine as well as colchicine, at a concentration of 100 microM, counteracted the effect of D2O. The enhanced release of histamine and LTC4 from basophils challenged with allergen was suppressed by Dimaprit, a histamine H2 receptor agonist, at a concentration required to inhibit the release by 50% of 5 X 10(-5) M for histamine and 10(-5) M for LTC4. These observations suggest that microtubules may be involved in LTC4 generation from immunologically stimulated basophils.
添加6% - 36%的氧化氘（D2O）会导致人嗜碱性粒细胞在过敏原或抗IgE刺激下白三烯C4（LTC4）生成呈剂量依赖性增加。与在正常缓冲液中孵育的对照组相比，在36% D2O存在的情况下，过敏原刺激的增强幅度为260±135%，抗IgE刺激的增强幅度为480±152%。在与过敏原孵育前洗涤细胞可完全逆转D2O对嗜碱性粒细胞LTC4生成的增加作用。浓度为100微摩尔的长春花碱以及秋水仙碱可抵消D2O的作用。组胺H2受体激动剂地马普明（Dimaprit）在抑制组胺释放50%所需浓度为5×10⁻⁵ M、抑制LTC4释放所需浓度为10⁻⁵ M时，可抑制过敏原刺激的嗜碱性粒细胞组胺和LTC4的释放增强。这些观察结果表明，微管可能参与免疫刺激的嗜碱性粒细胞生成LTC4的过程。
