Grant J A, Dupree E, Thueson D O
J Allergy Clin Immunol. 1977 Nov;60(5):306-11. doi: 10.1016/0091-6749(77)90110-5.
The release of histamine by normal human leukocytes (basophils) following in vitro challenge with activated complement (zymosan-treated serum) was previously reported. In this study, the effects of various pharmacologic agents on this release mechanism were compared with allergen-induced release of histamine. Colchicine and vinblastine antagonize the polymerization of tubulin to form microtubules, and both agents inhibited complement-and allergen-triggered release of histamine from basophils. Finally, treatment with cytochalasin B, a fungal product known to interfere with microfilament formatin, resulted in enhanced release of histamine from complement-treated basophils but no significant change in the percentage of histamine released from allergen-treated basophils. These findings suggest that microtubules and/or microfilaments are involved in complement-induced secretion of histamine by human basophils.
先前已有报道，正常人白细胞（嗜碱性粒细胞）在体外经活化补体（酵母聚糖处理的血清）刺激后会释放组胺。在本研究中，将各种药理剂对该释放机制的作用与变应原诱导的组胺释放进行了比较。秋水仙碱和长春碱可拮抗微管蛋白聚合成微管，这两种药剂均抑制了补体和变应原触发的嗜碱性粒细胞组胺释放。最后，用细胞松弛素B处理，这是一种已知会干扰微丝形成的真菌产物，结果导致经补体处理的嗜碱性粒细胞组胺释放增加，但经变应原处理的嗜碱性粒细胞释放组胺的百分比无显著变化。这些发现表明，微管和/或微丝参与了补体诱导的人嗜碱性粒细胞组胺分泌。
