Wu Guo-Lin, Pu Xing-Hong, Li Tian-Yi, Yu Guo-You, Lu Wen-Wen, Fan Yong-Sheng
Department of Chinese Medicine, First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310003, China.
Department of Internal Medicine, Qujing City Hospital of Traditional Chinese Medicine, Qujing 655000, China.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2013 Dec;33(12):1653-7.
To observe the effect of Yangyin Yiqi Huoxue Recipe (YYHR) on expression of interferon-gamma (IFN-gamma), IL-2, IL-4, IL-10, and immune balance of Th1/Th2 in serum and submaxillary glands of non-obese diabetic (NOD) mice with Sjogren's syndrome (SS), and to explore its mechanisms.
Totally 32 NOD mice were randomly into 4 groups, i.e., the model group, the Chinese medicine group [CM, administered with YYHR at the dose of 0.4 mL by gavage (100 g/kg)], the Western medicine group [WM group, administered with hydroxychloroquine 0.4 mL by gavage (60 mg/kg)], and the combined group [administered with YYHR (50 g/kg) and hydroxychloroquine (60 mg/kg) 0.4 mL by gavage], 8 mice in each group. Eight Balb/C mice were recruited as the normal control group. Mice were sacrificed to withdraw blood after 8 weeks. The submaxillary gland was excised. Serum levels of IFN-gamma, IL-2, IL-4, and IL-10 were detected by ELISA. Protein expression of IFN-gamma, IL-2, IL-4, and IL-10 in submaxillary glands was detected by SP method.
Compared with the normal group, levels of IFN-gamma, IL-2, IL-4, and IL-10 in serum and submaxillary glands all increased in the model group (P < 0.05). Levels of IFN-gamma and IL-10 in serum in the CM group and the combined group were lower than those of the WM group (P < 0.05). Serum levels of IFN-gamma and IL-2 in submaxillary glands in combined group were lower than those of the WM group (P < 0.05). The ratio of IFN-gamma/IL-4 in serum and submaxillary glands in the model group were higher than that of the rest groups (P < 0.05). Besides, it was the nearest to that of the normal group.
YYHR could decrease the levels of Th1 and Th2 related cytokines and the ratio of IFN-gamma/IL-4 in serum and submaxillary glands of NOD mice with SS. It could achieve therapeutic effects through adjusting immune balance of Th1/Th2 in SS mice.
观察养阴益气活血方（YYHR）对伴干燥综合征（SS）的非肥胖型糖尿病（NOD）小鼠血清及颌下腺中γ-干扰素（IFN-γ）、白细胞介素-2（IL-2）、白细胞介素-4（IL-4）、白细胞介素-10（IL-10）表达及Th1/Th2免疫平衡的影响，并探讨其作用机制。
将32只NOD小鼠随机分为4组，即模型组、中药组[CM组，按100 g/kg剂量以0.4 mL灌胃给予YYHR]、西药组[WM组，按60 mg/kg剂量以0.4 mL灌胃给予羟氯喹]和联合组[按50 g/kg剂量给予YYHR及60 mg/kg剂量给予羟氯喹，均以0.4 mL灌胃]，每组8只。另取8只Balb/C小鼠作为正常对照组。8周后处死小鼠取血，切除颌下腺。采用酶联免疫吸附测定（ELISA）法检测血清中IFN-γ、IL-2、IL-4和IL-10水平。采用免疫组化SP法检测颌下腺中IFN-γ、IL-2、IL-4和IL-10蛋白表达。
与正常组相比，模型组血清及颌下腺中IFN-γ、IL-2、IL-4和IL-10水平均升高（P < 0.05）。CM组和联合组血清中IFN-γ和IL-10水平低于WM组（P < 0.05）。联合组颌下腺中血清IFN-γ和IL-2水平低于WM组（P < 0.05）。模型组血清及颌下腺中IFN-γ/IL-4比值高于其余各组（P < 0.05）。此外，其最接近正常组。
YYHR可降低伴SS的NOD小鼠血清及颌下腺中Th1和Th2相关细胞因子水平及IFN-γ/IL-4比值。它可通过调节SS小鼠Th1/Th2免疫平衡发挥治疗作用。
