Chen Feng, Hou Shike, Li Qingsheng, Fan Haojun, Fan Rong, Xu Zhongwei, Zhala Gahu, Mai Xia, Chen Xiaoyi, Chen Xuyi, Liu Yingfu
Training Department, Logistics University of Chinese Armed Police Forces , Tianjin 300309, People's Republic of China.
Anal Chem. 2014 Oct 21;86(20):10021-4. doi: 10.1021/ac403872k. Epub 2014 Oct 3.
In this work, a novel enzyme-linked immunosorbent assay (ELISA) with a low limit of detection and high sensitivity was developed using atom transfer radical polymer (ATRP)-modified gold nanoparticles (AuNPs). Clear signal amplification was achieved by introducing an abundance of horseradish peroxidase (HRP) to the AuNPs, because of the ATRP modification. This result suggested that the new ELISA was able to detect antigens in complex mixtures, and the limit of detection (LOD) was lower than that of conventional ELISA by a factor of 81. The new ELISA strategy greatly decreased the LOD during analysis and exhibited excellent reproducibility, stability, and feasibility. Therefore, it is a promising technique with many potential applications in biochemistry and medical science research.
在这项工作中，利用原子转移自由基聚合（ATRP）修饰的金纳米颗粒（AuNPs）开发了一种具有低检测限和高灵敏度的新型酶联免疫吸附测定（ELISA）。由于ATRP修饰，通过向AuNPs引入大量辣根过氧化物酶（HRP）实现了明显的信号放大。该结果表明，新型ELISA能够检测复杂混合物中的抗原，其检测限（LOD）比传统ELISA低81倍。新型ELISA策略在分析过程中大大降低了LOD，并表现出优异的重现性、稳定性和可行性。因此，它是一种在生物化学和医学研究中有许多潜在应用的有前途的技术。
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