State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing 102206, P. R. China.
Anal Chem. 2013 Jul 2;85(13):6228-32. doi: 10.1021/ac401075u. Epub 2013 Jun 11.
Gold nanoparticles (GNPs) and graphene oxide (GO) sheets are excellent nano carriers in many analytical methods. In this study, a modified enzyme-linked immunosorbent assay (ELISA) strategy was developed using antibody-functionalized GO sheets and GNPs. This modification significantly reduced the limit of detection (LOD) and cost greatly of this assay. The applicability of the method was demonstrated by detecting HSP70 in a human serum sample. This result suggests that the 3G-ELISA method is feasible to detect an antigen in a complex mixture, and the LOD is up to 64-fold and the cost is as low as one-tenth of the conventional ELISA method.
金纳米粒子（GNPs）和氧化石墨烯（GO）片在许多分析方法中是极好的纳米载体。在本研究中，采用抗体功能化 GO 片和 GNPs 构建了一种改良的酶联免疫吸附测定（ELISA）策略。这种修饰显著降低了该测定的检测限（LOD）并大大降低了成本。通过检测人血清样品中的 HSP70 证明了该方法的适用性。结果表明，3G-ELISA 方法可用于检测复杂混合物中的抗原，检测限可达传统 ELISA 方法的 64 倍，成本仅为其十分之一。
