Fang Ye
Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, New York 14831, USA.
Comb Chem High Throughput Screen. 2014;17(7):566-78. doi: 10.2174/1386207317666140211100000.
Phenotypic screen holds great potential in the discovery of new small molecule probes and drugs, as it permits interrogating small molecules with native targets and pathways in model organisms and disease relevant cells. In recent years, label-free cell phenotypic profiling has emerged as an alternative for drug discovery. This paper provides the rationale for phenotypic screens, discusses the basics of label-free cell phenotypic profiling technologies, and provides some guidelines as to how to use these techniques to facilitate drug discovery.
表型筛选在发现新的小分子探针和药物方面具有巨大潜力，因为它允许在模式生物和疾病相关细胞中用天然靶点和信号通路来研究小分子。近年来，无标记细胞表型分析已成为药物发现的一种替代方法。本文阐述了表型筛选的基本原理，讨论了无标记细胞表型分析技术的基础，并就如何使用这些技术促进药物发现提供了一些指导方针。
