Ferrell Patrick I, Hexum Melinda K, Kopher Ross A, Lepley Michael A, Gussiaas Amanda, Kaufman Dan S
1 Stem Cell Institute, University of Minnesota , Minneapolis, Minnesota.
Stem Cells Dev. 2014 Jun 15;23(12):1355-63. doi: 10.1089/scd.2013.0497. Epub 2014 Mar 11.
To evaluate hematopoietic niche cell populations isolated from human embryonic stem cells (hESCs), we tested the ability of hESC-derived stromal lines to support CD34(+) umbilical cord blood (UCB)- and hESC-derived CD34(+)45(+) cells in long-term culture initiating cell (LTC-IC) assays. Specifically, these hematopoietic populations were cocultured with hESC-derived mesenchymal stromal cells (hESC-MSCs) and hESC-derived endothelial cells (hESC-ECs), and then assessed for their LTC-IC potential in comparison to coculture with bone marrow (BM)-derived MSCs and the mouse stromal line M2-10B4. We found that the hESC-derived stromal lines supported LTC-ICs from UCB similar to M2-10B4 cells and better than BM-MSCs. However, none of the stromal populations supported LTC-IC from hESC-derived CD34(+)45(+) cells. Engraftment data using the output from LTC-IC assays showed long-term repopulation (12 weeks) of NSG mice to correlate with LTC-IC support on a given stromal layer. Therefore, hESC-derived stromal lines can be used to efficiently evaluate putative hematopoietic stem/progenitor cells derived from hESCs or other cell sources.
为了评估从人胚胎干细胞（hESC）中分离出的造血微环境细胞群体，我们在长期培养起始细胞（LTC-IC）试验中测试了hESC来源的基质细胞系支持CD34(+)脐带血（UCB）细胞和hESC来源的CD34(+)45(+)细胞的能力。具体而言，将这些造血细胞群体与hESC来源的间充质基质细胞（hESC-MSC）和hESC来源的内皮细胞（hESC-EC）共培养，然后与与骨髓（BM）来源的MSC和小鼠基质细胞系M2-10B4共培养相比，评估它们的LTC-IC潜能。我们发现，hESC来源的基质细胞系支持来自UCB的LTC-IC，其效果与M2-10B4细胞相似，且优于BM-MSC。然而，没有一个基质细胞群体支持来自hESC来源的CD34(+)45(+)细胞的LTC-IC。使用LTC-IC试验结果的移植数据显示，NSG小鼠的长期再增殖（12周）与给定基质层上的LTC-IC支持相关。因此，hESC来源的基质细胞系可用于有效评估源自hESC或其他细胞来源的假定造血干/祖细胞。
