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焦碳酸二乙酯和高锰酸盐为抗肿瘤抗生素博来霉素和腐草霉素结合诱导的异常DNA构象提供了证据。

Diethylpyrocarbonate and permanganate provide evidence for an unusual DNA conformation induced by binding of the antitumour antibiotics bleomycin and phleomycin.

作者信息

Fox K R, Grigg G W

机构信息

Department of Physiology and Pharmacology, School of Biochemical and Physiological Sciences, University of Southampton, UK.

出版信息

Nucleic Acids Res. 1988 Mar 25;16(5):2063-75. doi: 10.1093/nar/16.5.2063.

DOI:10.1093/nar/16.5.2063
PMID:2451809
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC338199/
Abstract

DNA structural changes induced by bleomycin have been investigated using diethylpyrocarbonate and permanganate as probes under conditions in which the antibiotic binds to, but does not cut the DNA. Diethyl-pyrocarbonate shows an enhanced reaction with adenines in the presence of the antibiotic in the sequences GTA greater than GCA greater than GAA, on the 3' side of the drug cutting site (GPy). Permanganate ions display an enhanced reactivity at the second pyrimidine of the sequence GPyPy. The results are consistent with a model in which bleomycin distorts the structure of the base pair on the 3' side of its binding site.

摘要

在博来霉素与DNA结合但不切割DNA的条件下，使用焦碳酸二乙酯和高锰酸盐作为探针，研究了博来霉素诱导的DNA结构变化。在药物切割位点（GPy）的3'侧，焦碳酸二乙酯在抗生素存在的情况下，对序列GTA中的腺嘌呤反应增强，其次是GCA大于GAA。高锰酸根离子在序列GPyPy的第二个嘧啶处显示出增强的反应活性。结果与博来霉素使其结合位点3'侧碱基对结构扭曲的模型一致。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b62/338199/58f34849793b/nar00148-0199-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b62/338199/5fc88846649f/nar00148-0197-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b62/338199/36193e137a12/nar00148-0198-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b62/338199/58f34849793b/nar00148-0199-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b62/338199/5fc88846649f/nar00148-0197-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b62/338199/36193e137a12/nar00148-0198-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b62/338199/58f34849793b/nar00148-0199-a.jpg

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本文引用的文献

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The selective degradation of pyrimidines in nucleic acids by permanganate oxidation.通过高锰酸盐氧化对核酸中嘧啶进行选择性降解。
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Altered cleavage of DNA sequences by bleomycin and its deglycosylated derivative in the presence of actinomycin.在放线菌素存在的情况下，博来霉素及其去糖基化衍生物对DNA序列切割的改变。
Nucleic Acids Res. 1997 Apr 15;25(8):1516-22. doi: 10.1093/nar/25.8.1516.
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Interaction of bleomycin with a bent DNA fragment.博来霉素与弯曲DNA片段的相互作用。
Biochem J. 1992 Jun 15;284 ( Pt 3)(Pt 3):929-34. doi: 10.1042/bj2840929.
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Stoichiometry of DNA strand scission and aldehyde formation by bleomycin.
J Biol Chem. 1982 Aug 10;257(15):8612-4.
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Effects of O2 on the reactions of activated bleomycin.
J Biol Chem. 1982 Apr 10;257(7):3372-5.
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J Biol Chem. 1981 Nov 25;256(22):11636-44.
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