Department of Biochemistry, Orenburg State Medical Academy, Sovetskaya St., 6, Orenburg, 460014, Russia,
Biometals. 2014 Apr;27(2):293-303. doi: 10.1007/s10534-014-9712-0. Epub 2014 Feb 12.
The primary objective of the study was to estimate the effect of perinatal low-dose iron supplementation on diet-induced adipogenic action of a high-fat diet in the male offspring. The experimental group of pregnant dams was treated with drinking water containing 3 mg/l ferrous sulfate (FeSO4·7H2O) from the 2nd week of pregnancy till the end of lactation (the 21st day postpartum). The control group of dams obtained pure drinking water. The obtained male littermates were fed standard and high-fat diets (HFD) for 1 month. Animals' morphometric parameters as well as serum lipoprotein profile, glucose, insulin, adipokines and cytokines concentrations were estimated. Adipose tissue oxidative stress biomarkers were also measured. It is shown that HFD-fed perinatally iron treated rats had a significantly higher adipose tissue mass in comparison with HFD-control ones. The experimental iron-treated males were also characterized by increased serum glucose and insulin concentrations. Perinatally iron treated HFD-fed animals' leptin and proinflammatory cytokines concentrations exceeded the HFD-control values. Significant accumulation of free radical oxidation biomarkers is observed in adipose tissue samples. The lipoprotein spectra indicated initial atherogenic changes in the rats' serum. Taken together, the study suggests that iron takes part in the developmental programming of adipogenesis.
本研究的主要目的是评估围产期低剂量铁补充对高脂肪饮食诱导的雄性后代脂肪生成作用的影响。实验组的孕鼠从妊娠第 2 周开始至哺乳期结束（产后第 21 天）每天饮用含有 3mg/L 硫酸亚铁（FeSO4·7H2O）的水。对照组的孕鼠则饮用纯净水。获得的雄性同窝仔鼠在 1 个月内分别喂食标准饮食和高脂肪饮食（HFD）。评估了动物的形态计量学参数以及血清脂蛋白谱、血糖、胰岛素、脂肪因子和细胞因子浓度。还测量了脂肪组织氧化应激生物标志物。结果表明，与 HFD 对照组相比，经围产期铁处理的 HFD 喂养大鼠的脂肪组织质量显著增加。实验组的雄性还表现出血清葡萄糖和胰岛素浓度升高。与 HFD 对照组相比，经围产期铁处理的 HFD 喂养动物的瘦素和促炎细胞因子浓度升高。在脂肪组织样本中观察到自由基氧化生物标志物的大量积累。脂蛋白谱表明大鼠血清中存在初始动脉粥样硬化变化。总之，本研究表明铁参与了脂肪生成的发育编程。
