Tinkov Alexey A, Polyakova Valentina S, Nikonorov Alexandr A
Department of Biochemistry, Orenburg State Medical Academy, Orenburg, Russia.
Biometals. 2013 Jun;26(3):447-63. doi: 10.1007/s10534-013-9630-6. Epub 2013 May 9.
The primary objective of this research project is explore a possible adipogenic effect of iron and/or copper in albino Wistar rats kept on standard (STD) and high-fat (HFD) diets. The female Wistar rats in the study were divided into eight experimental groups (n = 6). Rats maintained on STD and HFD received 3 mg/l FeSO₄∙7H₂O, 4.88 mg/l CuSO₄ and a combination of 1.5 mg/l FeSO₄∙7H₂O and 2.44 mg/l CuSO₄ with drinking water. Control groups were kept on STD and HFD and received pure water without metal salts. Consumption of iron and copper in the groups of rats maintained on an STD did not produce a significant increase in weight, adipose tissue content or body mass index. However, the adipocyte size and infiltration were increased in the adipose tissue of STD-fed rats receiving a mixture of iron and copper with drinking water. The rats fed iron and copper and, especially, their combination on a HFD background had a significantly higher weight gain, adipose tissue content, morphometric parameters values and adipocyte size compared to STD- and HFD-fed controls. Iron and copper consumption produced their accumulation in the rats' adipose tissue. Moreover, the studied metals reduced adipose tissue concentration of chromium and vanadium. The lipoprotein profile and serum oxidative stress biomarkers were affected in the rats receiving the metals and STD. Hyperglycemia was observed in the rats receiving the studied metals on HFD-background. Based on the analysis of the test subjects, the study suggests that iron and copper administration, especially combined, may potentiate adipogenic effect of HFD.
本研究项目的主要目的是探究铁和/或铜对食用标准（STD）和高脂（HFD）饮食的白化Wistar大鼠可能产生的成脂作用。该研究中的雌性Wistar大鼠被分为八个实验组（n = 6）。食用STD和HFD的大鼠通过饮用水摄入3 mg/l硫酸亚铁∙7H₂O、4.88 mg/l硫酸铜以及1.5 mg/l硫酸亚铁∙7H₂O与2.44 mg/l硫酸铜的组合。对照组食用STD和HFD，并饮用不含金属盐的纯水。食用STD饮食的大鼠组摄入铁和铜后，体重、脂肪组织含量或体重指数并未显著增加。然而，饮用含铜铁混合物的STD饮食大鼠的脂肪组织中，脂肪细胞大小和浸润增加。与食用STD和HFD的对照组相比，食用铁和铜，尤其是同时食用两者的HFD饮食大鼠体重增加、脂肪组织含量、形态学参数值和脂肪细胞大小显著更高。铁和铜的摄入导致它们在大鼠脂肪组织中蓄积。此外，所研究的金属降低了脂肪组织中铬和钒的浓度。食用金属和STD的大鼠的脂蛋白谱和血清氧化应激生物标志物受到影响。在HFD饮食背景下食用所研究金属的大鼠出现了高血糖。基于对受试对象的分析，该研究表明，施用铁和铜，尤其是联合施用，可能会增强HFD的成脂作用。
