Bergfeld R
Botanisches Institut der Universität Freiburg i.Br., Freiburg i.Br., Deutschland.
Planta. 1968 Sep;81(3):274-9. doi: 10.1007/BF00391162.
Morphogenesis and differentiation of the young gametophytes (=sporelings) of Dryopteris filix-mas are controlled by light. Blue light leads to the formation of "normal" two-dimensional prothallia; under red light, however, the gametophytes grow as cellular filaments. Morphogenesis in blue light is connected with an increase in protein synthesis; in red light the protein content of the sporelings is markedly lower. The size of the chloroplasts is correlated with the protein content of the sporelings.In the present paper the diverse effect of chloramphenicol (CAP) and actidione (cycloheximide, ACT) was studied in connection with the formation of chloroplasts. ACT blocks the growth of the gametophytes, while the chloroplasts remain functional. On the other hand, CAP does not influence morphogenesis of the gametophytes. In particular the activity of the dividing apical cells remains untouched. Even when the light quality is changed during the development the corresponding specific effect of the light quality on morphogenesis is normal. The chloroplasts, however, become smaller, probably by inhibition of synthesis of structural proteins. But their synthetic activity is not completely suppressed. The specific blue or red light dependent morphogenesis is not changed, when protein synthesis in the chloroplasts is inhibited.
绵马鳞毛蕨幼配子体（= 孢子体）的形态发生和分化受光的控制。蓝光导致“正常”二维原叶体的形成；然而，在红光下，配子体以细胞丝状体的形式生长。蓝光下的形态发生与蛋白质合成的增加有关；在红光下，孢子体的蛋白质含量明显较低。叶绿体的大小与孢子体的蛋白质含量相关。在本文中，研究了氯霉素（CAP）和放线菌酮（环己酰亚胺，ACT）对叶绿体形成的不同影响。ACT 会阻碍配子体的生长，而叶绿体仍保持功能。另一方面，CAP 不影响配子体的形态发生。特别是分裂顶端细胞的活性不受影响。即使在发育过程中改变光照质量，光照质量对形态发生的相应特定影响仍是正常的。然而，叶绿体可能由于结构蛋白合成受到抑制而变小。但其合成活性并未完全被抑制。当叶绿体中的蛋白质合成受到抑制时，特定的蓝光或红光依赖的形态发生不会改变。
