Laboratory for Cell Physiology, Growth and Development, Cornell University, Ithaca, N. Y..
Planta. 1968 Dec;81(4):351-71. doi: 10.1007/BF00398021.
Knowing that the element Fe essentially triggers the action of the coconut milk stimulus for the growth of carrot explants and thereafter interacts with added Mo and Mn, the effects of these trace elements on growth (fresh and dry weight) and metabolism of both nitrogenous and nitrogen-free metabolites have been determined. An outstanding effect of Fe is to determine the level of protein synthesized, and the combination of Mo with Fe increases both protein and the content of non-protein nitrogen compounds. The role of Mn seems to reside in the mobilization of the soluble (non-protein) nitrogen compounds but its effects, which tend to compete with Fe in combination with Mo, tend to divert these compounds from protein synthesis. The element Fe appears again as a key element which determines the linkage between the use of carbon from carbohydrate and its entry into nitrogen metabolism: this has been shown by the use of (14)C-fructose as the source. Whereas Fe promotes the use of (14)C from fructose and directs it into protein, neither Mo nor Mn could achieve this either separately or in combination. The paper presents both the individual effects due to the trace elements and their interactions when supplied in combination. Stress is laid upon the need to consider the effects which are due to the inorganic elements in combination with the componenents of such growth-promoting systems as those present in coconut milk. These interactions are illustrated by polygonal diagrams (Figs. 3, 4 and 5). The point is made that any of several single entities of such an interacting complex may be in a given case rendered limiting, and the consequences of this fact for the concept of kinins or cell division factors are outlined. Any concept that requires cell division to be mediated solely by a given kind of chemical substance, or cell-division factor, would on the evidence here given present problems.
已知元素 Fe 基本上会引发椰子奶刺激物对胡萝卜外植体生长的作用，随后与添加的 Mo 和 Mn 相互作用，我们已经确定了这些微量元素对生长（鲜重和干重）和含氮和不含氮代谢物代谢的影响。Fe 的突出作用是确定合成蛋白质的水平，Mo 与 Fe 的结合增加了蛋白质和非蛋白氮化合物的含量。Mn 的作用似乎在于使可溶性（非蛋白）氮化合物移动，但它的作用与 Fe 与 Mo 结合时会产生竞争，从而使这些化合物从蛋白质合成中转移。Fe 再次作为一个关键元素出现，决定了碳水化合物中的碳与氮代谢进入的联系：这是通过使用 (14)C-果糖作为来源证明的。虽然 Fe 促进了 (14)C 从果糖中的利用，并将其导向蛋白质，但 Mo 和 Mn 都不能单独或联合实现这一点。本文介绍了微量元素的单独作用及其在组合供应时的相互作用。强调了需要考虑由于无机元素与促进生长系统的成分（如椰子奶中存在的成分）结合而产生的影响。这些相互作用通过多边形图（图 3、4 和 5）来说明。指出的是，在给定情况下，这种相互作用复杂的任何几个单一实体都可能受到限制，并且这一事实对细胞分裂因子或激肽的概念的后果进行了概述。任何需要细胞分裂仅仅由某种化学物质或细胞分裂因子来介导的概念，根据这里给出的证据，都会出现问题。
