Laboratory for Cell Physiology, Growth and Development Cornell University, Ithaca, N.Y..
Planta. 1968 Dec;81(4):333-50. doi: 10.1007/BF00398020.
The effects of Fe, Mo and Mn on the growth of explants drawn from different carrot clones were investigated. The explants were cultured aseptically on purified basal media supplemented with coconut milk. The two components of growth, i.e. cell division and cell enlargement, seem to respond differentially to the trace elements in question. Fe plays the key role and acts as a "trigger" of the action of coconut milk in stimulating cell division. Neither Mo nor Mn could replace iron in this respect. Mo and Mn acting separately tend to foster growth by cell enlargement to a different degree and at different concentrations. However, when Mo and Mn were added together to a medium containing iron, they seem to interact and stimulate growth by cell division and cell, enlargement still further. The suggestion is made that the behavior of the explants from different clones may have been responsive to differences in the functional Fe/Mn ratio which also involves endogenous levels of trace elements as well as the exogenous levels furnished in the medium.Thus, the element Fe emerges as the key trace element which interacts with the factors present in coconut milk to induce growth in the otherwise quiescent carrot tissue.
研究了 Fe、Mo 和 Mn 对不同胡萝卜克隆体外植体生长的影响。外植体在添加椰子乳的纯化基础培养基上进行无菌培养。这两个生长组成部分，即细胞分裂和细胞增大，似乎对所研究的微量元素有不同的反应。Fe 起着关键作用，并充当椰子乳刺激细胞分裂作用的“触发”因子。在这方面，Mo 和 Mn 都不能替代铁。Mo 和 Mn 单独作用时，在不同浓度下以不同程度促进细胞增大生长。然而，当 Mo 和 Mn 添加到含有铁的培养基中时，它们似乎相互作用并进一步通过细胞分裂和细胞增大刺激生长。有人提出，不同克隆体的外植体的行为可能对功能性 Fe/Mn 比值的差异有反应，这也涉及到内源微量元素水平以及培养基中提供的外源水平。因此，Fe 元素作为关键的痕量元素与椰子乳中存在的因子相互作用，诱导原本静止的胡萝卜组织生长。
