Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, NL-9747 AG Groningen, The Netherlands.
Macromol Biosci. 2014 Feb;14(2):186-94. doi: 10.1002/mabi.201300273. Epub 2013 Oct 8.
The successful synthesis of amylose brushes via enzymatic "grafting from" polymerization and the detailed characterization of all synthetic steps by X-ray photoelectron spectroscopy (XPS) and spectroscopic ellipsometry measurements are reported. Au and Si surfaces are amino-functionalized with self-assembled monolayers (SAMs) of cystamine and 3-aminopropyldimethyethoxysilane (APDMES), respectively. Maltoheptaose is covalently attached to the amino-functionalized Au and Si surfaces via reductive amination. Amylose brushes are grown from maltoheptaose modified Au and Si surfaces with enzymatic polymerization using potato phosphorylase and Rabbit Muscle phosphorylase, as evidenced by spectroscopic ellipsometry and XPS measurements.
通过酶促“从”聚合成功合成直链淀粉刷，并通过 X 射线光电子能谱（XPS）和光谱椭圆测量对所有合成步骤进行详细表征。分别用胱胺和 3-氨丙基二乙氧基硅烷（APDMES）的自组装单层（SAM）将 Au 和 Si 表面氨基功能化。通过还原胺化将麦芽七糖共价连接到氨基功能化的 Au 和 Si 表面。通过使用马铃薯磷酸化酶和兔肌肉磷酸化酶的酶促聚合，从麦芽七糖修饰的 Au 和 Si 表面生长直链淀粉刷，这一点通过光谱椭圆测量和 XPS 测量得到证实。
