Panaccio M, Zalcberg J R, Thompson C H, Leyden M J, Sullivan J R, Lichtenstein M, McKenzie I F
Department of Pathology, University of Melbourne, Parkville, Victoria, Australia.
Immunol Cell Biol. 1987 Dec;65 ( Pt 6):461-72. doi: 10.1038/icb.1987.55.
The human transferrin receptor (TFR) which is present on dividing cells, many tumours and erythroid precursors is readily identified using specific monoclonal antibodies. A new anti-human TFR monoclonal antibody, HuLy-m9, is described and its distribution on cell lines, normal and tumour tissue was examined and compared with two other anti-TFR monoclonal antibodies, namely, OKT9 and 5E9. The three antibodies were shown to recognise different epitopes on the surface of the TFR and have different reactivities with in vitro cell lines. Peptide map analyses of the TFR recognised by each monoclonal antibody from the same cell line were identical; however, differences were observed between cell lines. 131I-radiolabelled HuLy-m9 was used to successfully localise a nasopharyngeal carcinoma in vivo.
存在于分裂细胞、许多肿瘤细胞及红系前体细胞上的人转铁蛋白受体（TFR），可使用特异性单克隆抗体轻易识别。本文描述了一种新的抗人TFR单克隆抗体HuLy-m9，并检测了其在细胞系、正常组织和肿瘤组织中的分布情况，同时与另外两种抗TFR单克隆抗体OKT9和5E9进行了比较。结果显示，这三种抗体识别TFR表面不同的表位，且对体外细胞系具有不同的反应性。来自同一细胞系的每种单克隆抗体所识别的TFR的肽图分析结果相同；然而，不同细胞系之间存在差异。131I放射性标记的HuLy-m9被成功用于在体内定位鼻咽癌。
