Xu Yunling, Nie Jing, Hou Junjie, Xiao Ling, Lv Pan
a Hubei Institute for Food and Drug Control , Wuhan , PR China and.
b College of Pharmaceutical Sciences, Hubei Chinese Medical University , Wuhan , Hubei Province , PR China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):257-8. doi: 10.3109/19401736.2014.883614. Epub 2014 Feb 13.
The complete mitochondrial genome (14,414 bp) of the blood-feeding leech Hirudo nipponia, which was an important natural medicinal resource, was sequenced and characterized. The genome encodes 13 protein-coding genes, 2 rRNAs and 22 tRNAs. The content of A + T was 72.60% for H. nipponia (31.69% A, 40.91% T, 15.45% G and 11.95% C). All protein-coding genes started with ATN except for nad3 and nad5, which used GTG as start codon. Eight protein-coding genes stopped with termination codon TAA. Five protein-coding genes used incomplete stop codon TA or T. The A + T-rich region was located between tRNA-Arg and tRNA-His with a length of 83 bp. This is the first report about completely sequenced mitochondrial genome from the family Hirudinidae. The complete mitochondrial genomes of H. nipponia would be useful for the exploration of Hirudinea polygenetic relationships.
吸血水蛭日本医蛭（Hirudo nipponia）是一种重要的天然药用资源，其完整线粒体基因组（14,414 bp）已被测序并进行了特征分析。该基因组编码13个蛋白质编码基因、2个rRNA和22个tRNA。日本医蛭的A + T含量为72.60%（A为31.69%，T为40.91%，G为15.45%，C为11.95%）。除nad3和nad5以GTG作为起始密码子外，所有蛋白质编码基因均以ATN起始。8个蛋白质编码基因以终止密码子TAA结束。5个蛋白质编码基因使用不完全终止密码子TA或T。富含A + T的区域位于tRNA-Arg和tRNA-His之间，长度为83 bp。这是关于水蛭科完全测序线粒体基因组的首次报道。日本医蛭的完整线粒体基因组将有助于探索蛭纲的多基因关系。
