东方鳎（Brachirus orientalis）的完整线粒体基因组
Complete mitogenome of the oriental sole Brachirus orientalis.
作者信息
Chen Xiao, Ai Weiming, Shi Xiaofang, Pan Lianghao
机构信息
a Department of Marine Biotechnology , School of Life Science, Wenzhou Medical University , Wenzhou , PR China and.
b Guangxi Key Lab for Mangrove Conservation and Utilization , Guangxi Mangrove Research Center, Guangxi Academy of Sciences , Beihai , PR China.
出版信息
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):527-8. doi: 10.3109/19401736.2014.905840. Epub 2014 Apr 8.
The complete mitochondrial genome of the oriental sole Brachirus orientalis was presented in this study. It is 16,600 bp in length, contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region. A total of 28 bp short overlaps and 23 bp non-coding intergenic spacers were found in the mitogenome. The overall base composition of the L-strand is 30.5% A, 28.7% C, 15.2% G and 25.6% T. Two start codons (ATG and GTG) and three stop codons (AGG, TAG and TAA/T) were found in the protein-coding genes. Twenty-two tRNA genes ranged from 66 bp to 75 bp. Since the tRNA-Ser2 lacks the dihydrouridine arm it can not fold into a typical cloverleaf structure. The control region demonstrates the highest A+T content (66.4%) and the lowest G content (11.8%) in the mitogenome.
本研究公布了东方鳎（Brachirus orientalis）的完整线粒体基因组。其长度为16,600 bp，包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个控制区。在该线粒体基因组中总共发现了28 bp的短重叠序列和23 bp的非编码基因间隔区。轻链的总体碱基组成为：A占30.5%、C占28.7%、G占15.2%、T占25.6%。在蛋白质编码基因中发现了两个起始密码子（ATG和GTG）和三个终止密码子（AGG、TAG和TAA/T）。22个tRNA基因的长度在66 bp至75 bp之间。由于tRNA-Ser2缺乏二氢尿嘧啶臂，它无法折叠成典型的三叶草结构。控制区在该线粒体基因组中显示出最高的A+T含量（66.4%）和最低的G含量（11.8%）。
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