Bayramov Shahniyar, Guliyev Novruz
Institute of Botany, Azerbaijan National Academy of Sciences, 40 Patamdar Shosse, AZ-1073 Baku, Azerbaijan.
Institute of Botany, Azerbaijan National Academy of Sciences, 40 Patamdar Shosse, AZ-1073 Baku, Azerbaijan.
Plant Physiol Biochem. 2014 Aug;81:61-6. doi: 10.1016/j.plaphy.2014.01.013. Epub 2014 Jan 28.
Regulation of Rubisco (D-ribulose-1,5-bisphosphate carboxylase/oxygenase activase (RCA) gene expression and polypeptide content were determined in Brachypodium distachyon leaves, stems and ear elements at different developmental stages under optimal growth conditions as well as under drought and salt stress conditions. B. distachyon leaf contains a much greater amount of Rubisco activase small (RCAS) isoform than the large one (RCAL) under optimal growth conditions. Increased levels of the RCAL isoform compared with the RCAS isoform were found in leaves and in green stems under salt and drought stress, respectively. Transcriptional levels of RCA are almost identical in different leaf positions. Short-term drought and salt stresses did not cause the impairment of RCA gene expression in early seedlings. But gradually increasing drought stress significantly decreased gene expression in early seedling samples. Amounts of the RCAS isoform were found to be more in different leaves of the plant compared with the RCAL isoform and their ratio was constant under normal condition. In green stems gene expression of RCA decreased under salt and drought stresses, although as it was in green leaves protein amounts of RCAL isoform increased compared with the RCAS isoform. All of the above described results clearly indicate that the accumulation of each RCA isoform is differentially regulated by developmental and environmental cues.
在最佳生长条件下以及干旱和盐胁迫条件下，测定了不同发育阶段的短柄草叶片、茎和穗部元件中核酮糖-1,5-二磷酸羧化酶/加氧酶活化酶（RCA）基因表达和多肽含量的调控情况。在最佳生长条件下，短柄草叶片中核酮糖-1,5-二磷酸羧化酶活化酶小（RCAS）同工型的含量比大（RCAL）同工型多得多。在盐胁迫和干旱胁迫下，叶片和绿色茎中分别发现RCAL同工型水平相对于RCAS同工型有所增加。RCA在不同叶位的转录水平几乎相同。短期干旱和盐胁迫不会导致早期幼苗中RCA基因表达受损。但逐渐增加的干旱胁迫显著降低了早期幼苗样本中的基因表达。与RCAL同工型相比，在该植物的不同叶片中发现RCAS同工型的含量更多，并且在正常条件下它们的比例是恒定的。在绿色茎中，RCA的基因表达在盐胁迫和干旱胁迫下降低，尽管与绿色叶片中一样，RCAL同工型的蛋白质含量相对于RCAS同工型增加。上述所有结果清楚地表明，每个RCA同工型的积累受到发育和环境线索的差异调节。
