Liu Ai-yun, Liu Dian-Ggang, Du Ya-ju, Pei Feng-hua, Yang Guang, Liu Bing-rong, Zhang Hui-tao, Wang Xin-hong, Fan Yu-jing, Chen Ying-zhun, Jiang Yang, Chen Jing
Department of Gastroenterology; Second Affiliated Hospital of Harbin Medical University; Harbin, Heilongjiang PR China.
Department of General Surgery; Xuan Wu Hospital; Capital Medical University; Beijing, PR China.
Cancer Biol Ther. 2014 May;15(5):489-95. doi: 10.4161/cbt.28016. Epub 2014 Feb 12.
NPRL2 is a tumor suppressor gene involved in the progression of human cancer. The present study investigated whether NPRL2 expression correlates with colorectal cancer (CRC) progression. Colorectal tissue and peripheral blood samples were obtained from 62 patients with CRC, 38 patients with colorectal adenomas and 51 normal controls. NPRL2 mRNA levels in tissue samples and blood were measured using quantitative real-time PCR. NPRL2 protein expression was determined by immunohistochemistry. NPRL2 protein expression in CRCs was significantly lower than in the adenomas or normal colorectal tissue. NPRL2 mRNA expression was significantly decreased in adenomas compared with normal controls (P<0.0001) and it was further decreased in colorectal tumors compared with adenomas (P<0.0001). NPRL2 mRNA levels expression correlated with tumor stage. In addition, NPRL2 mRNA levels in the blood correlated with the levels detected in tumors. Furthermore, receiver operating characteristic (ROC) analysis showed that NPRL2 expression in blood could distinguish colorectal adenomas and CRCs from normal controls. NPRL2 mRNA expression in CRC tumor tissues and peripheral blood correlated with colorectal tumor progression. Based on our findings, we can conclude that NPRL2 mRNA blood levels could be a potentially useful marker for the detection of early stage adenomas and CRCs.
NPRL2是一种参与人类癌症进展的肿瘤抑制基因。本研究调查了NPRL2表达是否与结直肠癌（CRC）进展相关。从62例CRC患者、38例大肠腺瘤患者和51例正常对照者中获取结直肠组织和外周血样本。使用定量实时PCR测量组织样本和血液中NPRL2 mRNA水平。通过免疫组织化学确定NPRL2蛋白表达。CRC中NPRL2蛋白表达明显低于腺瘤或正常结直肠组织。与正常对照相比，腺瘤中NPRL2 mRNA表达明显降低（P<0.0001），与腺瘤相比，结直肠肿瘤中NPRL2 mRNA表达进一步降低（P<0.0001）。NPRL2 mRNA水平表达与肿瘤分期相关。此外，血液中NPRL2 mRNA水平与肿瘤中检测到的水平相关。此外，受试者工作特征（ROC）分析表明，血液中NPRL2表达可将大肠腺瘤和CRC与正常对照区分开来。CRC肿瘤组织和外周血中NPRL2 mRNA表达与结直肠肿瘤进展相关。基于我们的研究结果，我们可以得出结论，NPRL2 mRNA血液水平可能是检测早期腺瘤和CRC的潜在有用标志物。
