Yogurtcu B, Hatemi I, Aydin I, Buyru N
Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University, Kocamustafapasa, Istanbul, Turkey.
Genet Mol Res. 2012 Sep 12;11(4):4810-6. doi: 10.4238/2012.September.12.3.
Genetic and epigenetic factors affecting DNA methylation and gene expression are known to be involved in the development of colon cancer, but the full range of genetic alterations and many key genes involved in the pathogenesis of colon cancer remain to be identified. NPRL2 is a candidate tumor suppressor gene identified in the human chromosome 3p21.3 region. We evaluated the role of this gene in the pathogenesis of colorectal cancer by investigating NPRL2 mRNA expression in 55 matched normal and tumor colon tissue samples using quantitative RT-PCR analysis. There was significantly decreased NPRL2 expression in 45% of the patients. Lower NPRL2 expression was observed significantly more frequently in poorly differentiated tumor samples than in highly or moderately differentiated tumors. We conclude that expression of NPRL2 contributes to progression of colon cancer.
已知影响DNA甲基化和基因表达的遗传和表观遗传因素参与结肠癌的发展，但仍有待确定参与结肠癌发病机制的全部基因改变和许多关键基因。NPRL2是在人类染色体3p21.3区域鉴定出的候选肿瘤抑制基因。我们通过定量RT-PCR分析研究55对匹配的正常和肿瘤结肠组织样本中NPRL2 mRNA的表达，评估该基因在结直肠癌发病机制中的作用。45%的患者中NPRL2表达显著降低。在低分化肿瘤样本中观察到NPRL2表达降低的频率明显高于高分化或中分化肿瘤。我们得出结论，NPRL2的表达促进结肠癌的进展。
