Tang Yongyong, Jiang Li, Tang Wei
Department of Urology, The First Affiliated Hospital, Chongqing Medical University, No.1 Medical College Road, Yuzhong District, Chongqing, 400016, The People's Republic of China,
Pathol Oncol Res. 2014 Oct;20(4):829-37. doi: 10.1007/s12253-014-9761-2. Epub 2014 May 1.
The tumor suppressor gene nitrogen permease regulator-like 2(NPRL2) NPRL2 expressed obviously in many normal human tissues, but reduced in expression in many human tumors significantly. In this study, we detected the expression of NPRL2 in 78 clear cell renal cell carcinoma (ccRCC) by immunohistochemistry and correlated it with clinicopathological parameters. Meanwhile, the function of NPRL2 in human ccRCC was further explored after transfected recombinant expressing plasmids pEGFP-N1-NPRL2 into human renal cancer 786-0 cells. NPRL2 protein showed high expression in 67 of 78 cases of adjacent normal tissues (85.9 %), which was significantly higher than that in ccRCC tissues (23/78, 29.5 %). Clinic pathological analysis showed that NPRL2 expression was significantly correlated with histological grade (P = 0.044), TNM stage (P = 0.025) and lymph node metastasis (P = 0.028). MTT assay demonstrated that NPRL2 could obviously inhibit renal cancer cell proliferation. Flow cytometric analysis revealed that NPRL2 could induce renal cancer cells apoptosis and arrest the cell cycle in G0/G1 phase. In conclusion, NPRL2 is closely correlated to unfavourable pathological, proliferation and apoptotic features in ccRCC.
肿瘤抑制基因氮通透酶调节因子样2（NPRL2）在许多正常人体组织中表达明显，但在许多人类肿瘤中的表达显著降低。在本研究中，我们通过免疫组织化学检测了78例透明细胞肾细胞癌（ccRCC）中NPRL2的表达，并将其与临床病理参数相关联。同时，将重组表达质粒pEGFP-N1-NPRL2转染到人肾癌细胞786-0中后，进一步探讨了NPRL2在人ccRCC中的功能。NPRL2蛋白在78例癌旁正常组织中的67例（85.9%）中高表达，显著高于ccRCC组织（23/78，29.5%）。临床病理分析表明，NPRL2表达与组织学分级（P = 0.044）、TNM分期（P = 0.025）和淋巴结转移（P = 0.028）显著相关。MTT试验表明，NPRL2可明显抑制肾癌细胞增殖。流式细胞术分析显示，NPRL2可诱导肾癌细胞凋亡并使细胞周期停滞在G0/G1期。总之，NPRL2与ccRCC中不良的病理、增殖和凋亡特征密切相关。
