Takahashi Azusa, Okazaki Yukako, Nakamoto Aika, Watanabe Sanae, Sakaguchi Hirohide, Tagashira Yukari, Kagii Atsuko, Nakagawara Shunji, Higuchi Ohki, Suzuki Takashi, Chiji Hideyuki
Research Faculty and Graduate School of Agriculture, Hokkaido University.
J Oleo Sci. 2014;63(3):201-9. doi: 10.5650/jos.ess13196. Epub 2014 Feb 13.
Haskap (Lonicera caerulea L.) fruit contains some bioactive phenolic phytochemicals, mainly cyanidin-3-glucoside (cy3-glc) and chlorogenic acid. The purpose of this study was to investigate the effects of anthocyanin-rich phenolic phytochemical (containing 13.2% anthocyanin) purified from a Haskap fruit (named Haskap phytochemical) on postprandial serum triglyceride and blood glucose levels. The Haskap phytochemical (containing cy 3-glc at 300 mg/kg of body weight) was administered orally to rats fasted for 24 h and 30 min later, a corn oil emulsion was administered to these rats. After the administration, serum triglyceride concentration was measured. An increase in serum triglyceride concentration and the AUC significantly lowered in the Haskap phytochemical-administered group than in the saline-administered group. To evaluate the effect of serum glucose levels, the Haskap phytochemical was orally administered to rats fasted for 24 h and sucrose solution (2 g/kg of body weight) was administered to these rats after 30 min. After the administration, blood glucose level was measured. The Haskap phytochemical significantly reduced the increase in blood glucose levels and AUC in the Haskap phytochemical-administered group than in the saline-administered group. Furthermore, to investigate the long-term effects of Haskap phytochemical intake, high-fat diet (HF diet) with 1.5% or 3.0% Haskap phytochemical was administered to rats for four weeks. The investigation of chronological changes in the serum components of the rats fed HF diets in addition to the administration of Haskap phytochemical showed that the increase in serum triglyceride concentrations, total cholesterol concentrations and blood glucose were significantly suppressed compared to the HF diet-fed control (HF-control). These results suggest that the decrease in postprandial blood lipids and blood glucose by short or long-term Haskap phytochemical ingestion is due to anthocyanin and other polyphenols contained in the Haskap phytochemical.
蓝靛果（蓝靛果忍冬）果实含有一些具有生物活性的酚类植物化学物质，主要是矢车菊素 - 3 - 葡萄糖苷（cy3 - glc）和绿原酸。本研究的目的是调查从蓝靛果果实中纯化得到的富含花青素的酚类植物化学物质（含13.2%花青素，命名为蓝靛果植物化学物质）对餐后血清甘油三酯和血糖水平的影响。将蓝靛果植物化学物质（含300 mg/kg体重的cy 3 - glc）口服给予禁食24小时的大鼠，30分钟后给这些大鼠灌胃玉米油乳液。给药后，测量血清甘油三酯浓度。与生理盐水给药组相比，蓝靛果植物化学物质给药组的血清甘油三酯浓度和曲线下面积显著降低。为评估对血糖水平的影响，将蓝靛果植物化学物质口服给予禁食24小时的大鼠，30分钟后给这些大鼠灌胃蔗糖溶液（2 g/kg体重）。给药后，测量血糖水平。与生理盐水给药组相比，蓝靛果植物化学物质给药组的蓝靛果植物化学物质显著降低了血糖水平的升高和曲线下面积。此外，为研究摄入蓝靛果植物化学物质的长期影响，给大鼠喂食含1.5%或3.0%蓝靛果植物化学物质的高脂饮食（HF饮食），持续四周。除给予蓝靛果植物化学物质外，对喂食HF饮食的大鼠血清成分随时间变化的研究表明，与喂食HF饮食对照（HF - 对照）相比，血清甘油三酯浓度、总胆固醇浓度和血糖的升高得到显著抑制。这些结果表明，短期或长期摄入蓝靛果植物化学物质导致餐后血脂和血糖降低是由于蓝靛果植物化学物质中含有的花青素和其他多酚类物质。
