Nishi Hiroyuki, Nagamatsu Kumi
Laboratory of Analytical Chemistry, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Yasuda Women's University.
Anal Sci. 2014;30(2):205-11. doi: 10.2116/analsci.30.205.
This article presents a mini-review of the recent results in the ultra high-performance liquid chromatography (UHPLC) separation of pharmaceuticals by our group. High performance UHPLC separation employing core-shell particle C18 columns was demonstrated. High performance (high theoretical plate number of approximately 20000/10 cm, low theoretical plate height of 5 μm) was obtained without any specific devices in the conventional HPLC apparatus, only through changing detector sampling times and the inner diameter of the connecting tube. High theoretical plate numbers with low column back pressure obtained by the core-shell particle columns enabled fast separation of the analytes. Methanol, which gives high column pressure drops in the reversed-phase mode HPLC compared with acetonitrile, can be used without any trouble. One analysis of the purity testing of diltiazem hydrochloride was performed within 100 s. One analysis in the photostability testing of mecobalamin (vitamin B12 analogue) was successful within 180 s.
本文对我们小组近期在超高效液相色谱（UHPLC）分离药物方面的成果进行了简要综述。展示了采用核壳颗粒C18柱的高效UHPLC分离。仅通过改变检测器采样时间和连接管内径，在常规HPLC仪器中无需任何特殊装置即可实现高效分离（理论塔板数约为20000/10 cm，理论塔板高度低至5μm）。核壳颗粒柱获得的高理论塔板数和低柱背压使得分析物能够快速分离。与乙腈相比，在反相模式HPLC中会导致高柱压降的甲醇也能毫无问题地使用。盐酸地尔硫䓬的纯度检测一次分析可在100秒内完成。甲钴胺（维生素B12类似物）光稳定性检测的一次分析在180秒内成功完成。
