Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
Miami Integrative Metabolomics Research Center, Miami, FL, USA.
Methods Mol Biol. 2024;2816:35-40. doi: 10.1007/978-1-0716-3902-3_4.
Sphingolipids, including sphingosine and sphinganine, are one of the major classes of lipids. They serve as constituents of cell membranes and lipid rafts and aid in the performance of cell-cell communication and adhesion. Abnormal levels of sphingolipids in the aqueous humor can indicate impaired sphingolipid metabolism and associated ocular pathologies. Sphingolipids can be extracted from the aqueous humor by the methyl-tert-butyl ether (MTBE) lipid extraction method and subsequently analyzed by liquid chromatography-mass spectrometry (LC-MS). This chapter describes a modified protocol for an MTBE lipid extraction from the aqueous humor, followed by analysis with ultrahigh-performance liquid chromatography-mass spectrometry (UHPLC-MS).
鞘脂类，包括神经酰胺和神经鞘氨醇，是脂质的主要类别之一。它们是细胞膜和脂筏的组成部分，有助于细胞间通讯和黏附。房水中鞘脂类的异常水平表明鞘脂代谢受损和相关的眼部病变。鞘脂类可以通过甲基叔丁基醚（MTBE）脂质提取方法从房水中提取，然后通过液相色谱-质谱（LC-MS）进行分析。本章描述了一种改良的 MTBE 从房水中提取脂质的方案，然后通过超高效液相色谱-质谱（UHPLC-MS）进行分析。
