Queen Mary College, London.
Planta. 1967 Dec;77(4):354-66. doi: 10.1007/BF00389320.
D-threo-chloramphenicol at concentrations ranging from 1000 to 3000 μg/ml stimulated the germination of the light-sensitive seeds of the lettuce (Lactuca sativa L.) varieties Attractie and Grand Rapids. This stimulatory effect of chloramphenicol was markedly temperature dependent, increasing with decrereasing temperature. Seeds showed little response to chloramphenicol at temperatures of 28°C and above except in the case of light treated Attractie seed. The failure of one batch of Grand Rapids seed to respond to chloramphenicol was associated with the low degree of dormancy in this batch.When the germination of half-seeds or intact excised embryos of Attractie seed was inhibited osmotically with 0.15 M NaCl a stimulatory response to chloramphenicol was obtained suggesting that the site of action was in the embryo itself.Other inhibitors of protein synthesis, cycloheximide, puromycin and p-fluorophenylalanine, did not stimulate germination. Cycloheximide at concentrations of 10 μg/ml and above inhibited germination whereas puromycin and p-fluorophenylalanine were relatively ineffective as germination inhibitors.
D-苏式-氯霉素在 1000 到 3000μg/ml 的浓度范围内能够刺激生菜（Lactuca sativa L.）品种 Attractie 和 Grand Rapids 的光敏种子发芽。氯霉素的这种刺激作用明显依赖于温度，随着温度的降低而增加。在 28°C 及以上的温度下，种子对氯霉素的反应很小，除了经过光处理的 Attractie 种子的情况。一批 Grand Rapids 种子对氯霉素没有反应，这与这批种子休眠程度低有关。当 Attractie 种子的半种子或完整切除的胚胎的萌发被 0.15 M NaCl 渗透抑制时，会对氯霉素产生刺激反应，这表明作用部位在胚胎本身。其他蛋白质合成抑制剂，如环己亚胺、嘌呤霉素和对氟苯丙氨酸，不能刺激萌发。环己亚胺在 10μg/ml 及以上的浓度下抑制萌发，而嘌呤霉素和对氟苯丙氨酸作为萌发抑制剂的效果相对较差。
